Isolation of variant lymphoma cells with reduced growth requirements for extracellular calcium and magnesium and enhanced oncogenicity

Autor:	J K, Brennan, M A, Lichtman, J K, Chamberlain, P, Leblond
Rok vydání:	1976
Předmět:	Lymphoma Immunology Neoplasms Experimental Cell Biology Hematology Biochemistry Cell Line Clone Cells Culture Media Rats Microscopy Electron Carcinogens Animals Calcium Magnesium Cell Aggregation
Zdroj:	Blood. 47:447-459
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v47.3.447.bloodjournal473447
Popis:	By exposing an established cell line of malignant mouse lymphocytic cells (L5178Y) to a culture environment low in calcium and magnesium, we have isolated and maintained in continuous culture a variant population with reduced growth requirements for the cations. The variant cells are larger, have enhanced aggregability, tend to form dispersed colonies in semisolid medium, and have increased oncogenicity. Variants and their progenitors share similar morphology, maximum proliferative rate, and stability of phenotype. The significance of these findings is discussed and an analogy suggested between the selective influence of calcium and magnesium deprivation in vitro and the evolution of thymic dysplasias in divalent cation- deprived rodents.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cdde979ed19b9b689549536047a231f https://doi.org/10.1182/blood.v47.3.447.bloodjournal473447 Zobrazit plný text záznamu