In Vitro and In Vivo Models for Studying SARS-CoV-2, the Etiological Agent Responsible for COVID-19 Pandemic
| Autor: | Jéssica Catarine Frutuoso do Nascimento, Ronaldo N. de Oliveira, Lindomar Pena, Murilo V. Silva, Rafael B Rosa, Willyenne Marilia Dantas |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell lcsh:QR1-502 Review In Vitro Techniques Biology Virus Replication lcsh:Microbiology Cell Line 03 medical and health sciences 0302 clinical medicine In vivo Virology Pandemic medicine Animals Humans Pandemics in vitro models Lung SARS-CoV-2 COVID-19 respiratory system In vitro Disease Models Animal 030104 developmental biology Infectious Diseases medicine.anatomical_structure Viral replication Cell culture in vivo models Immortalised cell line 030217 neurology & neurosurgery |
| Zdroj: | Viruses, Vol 13, Iss 379, p 379 (2021) Viruses |
| ISSN: | 1999-4915 |
| Popis: | The emergence and rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prompted the scientific community to rapidly develop in vitro and in vivo models that could be applied in COVID-19 research. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures derived from lung, alveoli, bronchi, and other organs. Although cell-based systems are economic and allow strict control of experimental variables, they do not always resemble physiological conditions. Thus, several in vivo models are being developed, including different strains of mice, hamsters, ferrets, dogs, cats, and non-human primates. In this review, we summarize the main models of SARS-CoV-2 infection developed so far and discuss their advantages, drawbacks and main uses. |
| Databáze: | OpenAIRE |
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