The energy-linked transhydrogenase reaction in respiratory mutants of Escherichia coli K12
| Autor: | Frank Gibson, N. A. Newton, J. D. Butlin, Graeme B. Cox |
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| Rok vydání: | 1971 |
| Předmět: |
History
Pyridines Ubiquinone Mutant Biology medicine.disease_cause Education Electron Transport chemistry.chemical_compound Adenosine Triphosphate medicine Escherichia coli Piericidin A Magnesium Respiratory system Adenosine Triphosphatases Adenosine triphosphatase Quinones Articles NAD Computer Science Applications A-site Membrane chemistry Biochemistry Mutation Calcium Energy source Oxidoreductases |
| Zdroj: | The Biochemical journal. 125(2) |
| ISSN: | 0264-6021 |
| Popis: | 1. Energy-linked and non-energy-linked transhydrogenase activities were assayed in membrane preparations from normal Escherichia coli K 12 and from various mutant strains. 2. The energy-linked transhydrogenase, which uses ATP as energy source, was dependent for activity on the presence of a functional Mg2++Ca2+-stimulated adenosine triphosphatase. 3. Neither of the quinones formed by E. coli, namely ubiquinone-8 and menaquinone-8, was required for normal ATP-dependent energy-linked transhydrogenase activity. 4. The energy-linked transhydrogenase was inhibited by piericidin A at a site unrelated to the sites of inhibition of the electron-transport chain by piericidin A. |
| Databáze: | OpenAIRE |
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