MOESM5 of The roles of kinetochore of micronucleus in mitosis of HeLa cells: a live cell imaging study

Autor: Erkang Jiang, Lianping Wei, Tao, Fang, Yu, Mei, Wang, Shu, Xiuhong Zhou, Daxiang Li, Zhongwen Xie
Rok vydání: 2019
DOI: 10.6084/m9.figshare.9247577
Popis: Additional file 5: Figure S3. DNA degradation was not obvious in Hela Cells containing mainly K−MNs and K+MNs. Cells were exposed to actinomycin D and colcemid and for 24 h, and DNA were isolated from each treatment for gel electrophoresis as described in “Methods” section. (1) 100 bp DNA ladder marker (Takara Corp.); (2) Control; (3) Cells treated with 150 ng/mL actinomycin D; (4) Cells treated with 15 ng/mL actinomycin D; (5) Cells treated with 25 ng/mL colcemid. Results suggested that there was no DNA degradation in control cells. DNA degradation was obvious in the high concentration of actinomycin D treatment (150 ng/mL), while slight DNA degradation occurred in the colcemid and low concentration actinomycin D (15 ng/mL) treatment cells.
Databáze: OpenAIRE