Comparison of the Vaginal Environment in Rhesus and Cynomolgus Macaques Pre and Post Lactobacillus Colonization
| Autor: | Deborah J. Anderson, Hojin Moon, Chunxia Zhao, Francois Villinger, Michael W. Cho, Patricia Oviedo-Moreno, Thomas R. Moench, Gregory J. Daggett, Fawn Connor-Stroud |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Human immunodeficiency virus (HIV) HIV Infections medicine.disease_cause Article 03 medical and health sciences 0302 clinical medicine Reference Values Lactobacillus medicine Animals Humans Colonization 030212 general & internal medicine Pre and post Vaginal route General Veterinary biology biology.organism_classification medicine.disease Macaca mulatta Disease Models Animal Macaca fascicularis 030104 developmental biology Immunology Vagina Animal Science and Zoology Female Nugent score Bacterial vaginosis |
| Popis: | Background Rhesus and cynomologus macaques are valuable animal models for the study of human immunodeficiency virus (HIV) prevention strategies. However, for such studies focused on the vaginal route of infection, differences in vaginal environment may have deterministic impact on the outcome of such prevention, providing the rationale for this study. Methods We tested the vaginal environment of rhesus and cynomolgus macaques longitudinally to characterize the normal microflora based on Nugent scores and pH. This evaluation was extended after colonization of the vaginal space with Lactobacilli in an effort to recreate NHP models representing the healthy human vaginal environment. Results and Conclusion Nugent scores and pH differed significantly between species, although data from both species were suggestive of stable bacterial vaginosis. Colonization with Lactobacilli was successful in both species leading to lower Nugent score and pH, although rhesus macaques appeared better able to sustain Lactobacillus spp over time. |
| Databáze: | OpenAIRE |
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