Ciprofloxacin Resistance and Its Molecular Mechanism inCampylobacterspp. Isolated in Kuwait

ATT) gyrA mutation and three resistant strains of C. jejuni studied had Thr-86-to-Ile (ACA--> ATA) gyrA mutation. In addition, all the three C. jejuni strains had silent mutations. Thus, ciprofloxacin is of limited use for treatment in Kuwait and MAMA PCR is a useful assay to study gyrA mutation. Because Kuwait has a large expatriate population of workers, it can be a focus of spread of antimicrobial resistance. -->

ISSN:	1931-8448 1076-6294
DOI:	10.1089/mdr.2005.11.266
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cd5ede8ebc5888d414fc09d60487134 https://doi.org/10.1089/mdr.2005.11.266
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi.dedup.....1cd5ede8ebc5888d414fc09d60487134
Autor:	Lalitha Neil, Aleyamma A. Pazhoor, Shilpa Haridas, M. John Albert, Vincent O. Rotimi, Islam Khan
Rok vydání:	2005
Předmět:	DNA Bacterial Microbiology (medical) Silent mutation Immunology Population Erythromycin Drug resistance Biology medicine.disease_cause Polymerase Chain Reaction Microbiology law.invention Antibiotic resistance Ciprofloxacin law Drug Resistance Bacterial medicine education Polymerase chain reaction Pharmacology education.field_of_study Campylobacter biochemical phenomena metabolism and nutrition bacterial infections and mycoses Virology Kuwait DNA Gyrase Mutation medicine.drug
Zdroj:	Microbial Drug Resistance. 11:266-270
ISSN:	1931-8448 1076-6294
DOI:	10.1089/mdr.2005.11.266
Popis:	Campylobacter spp. are an important cause of diarrhea in Kuwait. Because susceptibility data for ciprofloxacin and erythromycin, the two recommended drugs for treatment, are not available for this part of the world, 64 Campylobacter spp. isolates obtained from human diarrheal stools in Kuwait during 2000--2003 were studied for susceptibility to these antimicrobials by E-test. The utility of a simple mismatch amplification mutation assay (MAMA) PCR to detect base substitution in the gyrA gene mediating resistance to ciprofloxacin was also explored. Approximately, 53% (34/64) of the isolates were resistant to ciprofloxacin (MIC, 4-64 microg/ml) and 5% (3/64) to erythromycin (MIC>256 microg/ml). MAMA PCR showed a Thr-86-to-Ile mutation in gyrA gene of 23/26 ciprofloxacin-resistant C. jejuni, and in all resistant C. coli. Sequencing of PCR product showed that two resistant strains of C. coli studied had Thr-86-to-Ile (ACT--> ATT) gyrA mutation and three resistant strains of C. jejuni studied had Thr-86-to-Ile (ACA--> ATA) gyrA mutation. In addition, all the three C. jejuni strains had silent mutations. Thus, ciprofloxacin is of limited use for treatment in Kuwait and MAMA PCR is a useful assay to study gyrA mutation. Because Kuwait has a large expatriate population of workers, it can be a focus of spread of antimicrobial resistance.
Databáze:	OpenAIRE
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