Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope
| Autor: | Ricardo Andrez Machado de Ávila, Carolina K. Miyazaki, Mariana C. Duarte, Eduardo A.F. Coelho, Bruno Mendes Roatt, Vívian T. Martins, Lucas M.O. Santos, Paula Melo de Abreu Vieira, Amanda Christine Da Silva Kursancew, Fernanda F. Ramos, Fernanda Ludolf, Daniel Menezes-Souza, Daniel Dias, Marcella Rezende Rodrigues, Denise Utsch Gonçalves, Jamil S. Oliveira |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Chagas disease Ehrlichia canis Immunology Antigens Protozoan Enzyme-Linked Immunosorbent Assay Epitope law.invention 03 medical and health sciences Dogs 0302 clinical medicine Predictive Value of Tests law Prohibitins medicine Animals Humans Immunology and Allergy Serologic Tests Dog Diseases Leishmania infantum biology business.industry Reproducibility of Results Leishmaniasis biology.organism_classification medicine.disease Molecular biology Repressor Proteins 030104 developmental biology Visceral leishmaniasis Case-Control Studies Recombinant DNA biology.protein Epitopes B-Lymphocyte Leishmaniasis Visceral Antibody business Leishmania donovani 030215 immunology |
| Zdroj: | Journal of Immunological Methods. 474:112641 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2019.112641 |
| Popis: | In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study analyzed serum samples from cutaneous (n = 20) or mucosal (n = 39) leishmaniasis patients, and from Chagas disease (CD) patients (n = 8) and non-infected patients (n = 45). For CVL diagnosis, serum samples from asymptomatic (n = 14), symptomatic (n = 71), non-infected (n = 116), and Leish-Tec®-vaccinated (n = 79) dogs were examined, as well as T. cruzi (n = 11) and Ehrlichia canis (n = 10) infected animals. An indirect ELISA method using rProhibitin showed diagnostic sensitivity and specificity values of 91.76% and 89.91%, respectively. L. infantum SLA showed 86.11% and 48.24% of specificity and sensitivity, respectively, for CVL serodiagnosis, and 98.31% and 84.91% sensitivity and specificity, respectively for TL diagnosis. L. braziliensis SLA showed 75.47% and 83.05% of specificity and sensitivity, respectively, for TL diagnosis. The synthetic peptide showed a better result in TL than in CVL diagnosis. In conclusion, preliminary results suggest that the detection of antibodies against the rProhibitin protein and the synthetic peptide improves the serodiagnosis of TL and CVL. |
| Databáze: | OpenAIRE |
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