High-performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats
| Autor: | Connie M. Remsberg, Shyam S. Sablani, Neal M. Davies, Karina R. Vega-Villa, Rebecca M. Bertram, Jody K. Takemoto |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Calibration curve Formic acid Clinical Biochemistry Urine Biochemistry High-performance liquid chromatography Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Pharmacokinetics Limit of Detection Drug Discovery Stilbenes Animals Molecular Biology Chromatography High Pressure Liquid Pharmacology Detection limit Chromatography Chemistry Plant Extracts Extraction (chemistry) General Medicine Oxyresveratrol Rats Dietary Supplements |
| Zdroj: | Biomedical chromatography : BMC. 24(5) |
| ISSN: | 1099-0801 |
| Popis: | A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was |
| Databáze: | OpenAIRE |
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