Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi
| Autor: | Samuel Goldenberg, Gisele Fernanda Assine Picchi, Priscila M. Hiraiwa, Lysangela R. Alves, Beatriz S. Borges, Bruno Accioly Alves Romagnoli |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chagas disease Veterinary (miscellaneous) Trypanosoma cruzi 030106 microbiology Biology Genome 03 medical and health sciences parasitic diseases medicine CRISPR Gene Genetics Nuclease Cas9 biology.organism_classification medicine.disease Protozoan parasite 030104 developmental biology Infectious Diseases Insect Science biology.protein Parasitology CRISPR-Cas Systems Genetic Engineering |
| Zdroj: | Acta tropica. 178 |
| ISSN: | 1873-6254 |
| Popis: | Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions of individuals around the world. Although it has been known for more than a century, the study of T. cruzi has been a challenge, particularly due to the scarcity of tools for genome inquiries. Recently, strategies have been described allowing gene disruption in T. cruzi by the CRISPR/Cas9 nuclease system. Although these strategies demonstrated success in deleting some genes, several aspects could be improved to increase the efficiency of the CRISPR/Cas9 system in T. cruzi. Here, we report a strategy, based on adaptations and improvements of the two previously described systems, that results in efficient gene disruption that can be applied to any target, including the study of essential genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect