Changes in equine endometrial oestrogen receptor and progesterone receptor mRNAs during the oestrous cycle, early pregnancy and after treatment with exogenous steroids
| Autor: | M. H. Adams, K. J. McDowell, Simpson Ks, C. Y. Adam |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
endocrine system
Embryology medicine.medical_specialty medicine.drug_class media_common.quotation_subject Radioimmunoassay Uterus Gestational Age Biology Endometrium Endocrinology Estrus Pregnancy Internal medicine Progesterone receptor medicine Animals Horses RNA Messenger Cloning Molecular Ovulation Progesterone reproductive and urinary physiology media_common Estrous cycle Analysis of Variance Estradiol Obstetrics and Gynecology Cell Biology medicine.anatomical_structure Receptors Estrogen Reproductive Medicine Estrogen In utero Autoradiography Pregnancy Animal Electrophoresis Polyacrylamide Gel Female Receptors Progesterone Estrogen receptor alpha |
| Zdroj: | Reproduction. 117:135-142 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.1170135 |
| Popis: | Two experiments were performed to determine changes in the abundance of oestrogen and progesterone receptor (ER alpha and PR) mRNAs in equine endometrium during the oestrous cycle and early pregnancy, and under the influence of exogenous steroids. In Expt 1, endometrial biopsies were obtained from non-mated mares during oestrus and at days 5, 10 and 15 after ovulation, and from pregnant mares at days 10, 15 and 20 after ovulation. There were overall effects of day on the abundance of ER alpha (P = 0.0001) and PR (P = 0.0014) mRNAs. The amount of ER alpha mRNA decreased at day 10 of pregnancy, and PR mRNA was reduced at day 5 in non-mated mares and at day 15 of pregnancy, compared with oestrous values. Experiment 2 was conducted to determine the effects of exogenous steroids on endometrial ER alpha and PR mRNAs. Endometrial biopsies were obtained from 19 anoestrous mares that had been treated with vehicle, oestradiol, progesterone, or oestradiol followed by progesterone for either a short or a long duration. The steroid treatment affected the abundance of ER alpha mRNA (P = 0.0420), which was higher (P < 0.05) in the oestradiol group than in the group treated with oestradiol followed by long duration progesterone. The steroid treatment did not affect the abundance of PR mRNA. These results demonstrate that the amount of steroid receptor mRNA changes with the fluctuating steroid environment in the uterine endometrium of cyclic and early pregnant mares, and that the duration of progesterone dominance may affect ER alpha gene expression. In addition, factors other than steroids may regulate ER alpha and PR gene expression in equine uterine endometrium. |
| Databáze: | OpenAIRE |
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