Human decellularized adipose tissue scaffold as a model for breast cancer cell growth and drug treatments
| Autor: | Zhao Huang, Ningyan Zhang, Qixu Zhang, Zhiqiang An, Weixu Meng, Lina W. Dunne, Xuejun Fan |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Cell Survival Biophysics Mice Nude Adipose tissue Antineoplastic Agents Breast Neoplasms Bioengineering Cell morphology Biomaterials Extracellular matrix Biomimetics Cell Movement Cell Line Tumor Cell Adhesion medicine Animals Humans Regeneration Cell Proliferation Mice Inbred BALB C Tumor microenvironment Matrigel Decellularization Tissue Engineering Tissue Scaffolds Cancer Lapatinib medicine.disease Immunohistochemistry Extracellular Matrix Adipose Tissue Mechanics of Materials Cancer cell MCF-7 Cells Microscopy Electron Scanning Quinazolines Ceramics and Composites Cancer research Female Neoplasm Transplantation |
| Zdroj: | Biomaterials. 35:4940-4949 |
| ISSN: | 0142-9612 |
| Popis: | Human adipose tissue extracellular matrix, derived through decellularization processing, has been shown to provide a biomimetic microenvironment for adipose tissue regeneration. This study reports the use of human adipose tissue-derived extracellular matrix (hDAM) scaffolds as a three-dimensional cell culturing system for the investigation of breast cancer growth and drug treatments. The hDAM scaffolds have similar extracellular matrix composition to the microenvironment of breast tissues. Breast cancer cells were cultured in hDAM scaffolds, and cell proliferation, migration, morphology, and drug responses were investigated. The growth profiles of multiple breast cancer cell lines cultured in hDAM scaffolds differed from the growth of those cultured on two-dimensional surfaces and more closely resembled the growth of xenografts. hDAM-cultured breast cancer cells also differed from those cultured on two-dimensional surfaces in terms of cell morphology, migration, expression of adhesion molecules, and sensitivity to drug treatment. Our results demonstrated that the hDAM system provides breast cancer cells with a biomimetic microenvironment in vitro that more closely mimics the in vivo microenvironment than existing two-dimensional and Matrigel three-dimensional cultures do, and thus can provide vital information for the characterization of cancer cells and screening of cancer therapeutics. |
| Databáze: | OpenAIRE |
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