Identification of nodulation promoter (nod-box) regions of Rhizobium galegae
| Autor: | Ari-Matti Saren, Lars Paulin, Leena Suominen, Eva Tas, Aimo Saano, Kristina Lindström |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping medicine.disease_cause Microbiology Genome Plant Roots Rhizobium leguminosarum Rhizobium galegae 03 medical and health sciences Nitrogen Fixation Sequence Homology Nucleic Acid Genetics medicine Promoter Regions Genetic Symbiosis Molecular Biology Gene 030304 developmental biology 0303 health sciences Sinorhizobium meliloti biology Base Sequence 030306 microbiology food and beverages Nif gene Sequence Analysis DNA biochemical phenomena metabolism and nutrition biology.organism_classification Cosmids Genes Bacterial Cosmid bacteria Rhizobium |
| Zdroj: | FEMS microbiology letters. 177(2) |
| ISSN: | 0378-1097 |
| Popis: | A hybridisation analysis of a genomic clone library of Rhizobium galegae HAMBI 1174 located four EcoRI fragments homologous to the nod-box promoter sequence of Sinorhizobium meliloti in two separate gene regions. Two of the five nod-boxes detected in the R. galegae genome were carried on a single cosmid clone, pRg30, upstream from the nodABCIJ and nodF genes, whereas the other three nod-boxes were carried on a different cosmid clone, pRg10. Hybridisations with various nod gene probes from S. meliloti and Rhizobium leguminosarum species detected a nodD homolog in pRg10. The sequence data obtained from regions adjacent to each nod-box in pRg10 confirmed the presence of a second nodD in the R. galegae genome and, in addition, revealed the presence of nodN, nodU, dctA nifH and nifQ-like genes in pRg10. Thus, by using a promoter-specific nod-box probe we could identify a new region carrying genes involved in nitrogen fixation and host specificity functions. |
| Databáze: | OpenAIRE |
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