Interactive effects of temperature and salinity on the growth and cytotoxicity of the fish-killing microalgal species Heterosigma akashiwo and Pseudochattonella verruculosa
| Autor: | Alondra Sandoval-Sanhueza, Alejandra Aguilera-Belmonte, Leila Basti, Rosa I. Figueroa, Carlos Molinet, Gonzalo Álvarez, Sandra Oyanedel, Pilar Riobó, Guido Mancilla-Gutiérrez, Patricio A. Díaz |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
fish
Temperatures Salinity HAB HAB temperatura salinidad ictiotoxicidad salmón Ichthyotoxicity Temperature Fish embryo cell lines Aquatic Science Oceanography marine pollution Centro Oceanográfico de Vigo Microalgae rights Animals cytotoxicity pollution Medio Marino Chinook salmon Homicide Stramenopiles |
| Zdroj: | e-IEO. Repositorio Institucional Digital de Acceso Abierto del Instituto Español de Oceanografía instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | 12 pages, 7 figures, 3 tables Fish-killing blooms of Heterosigma akashiwo and Pseudochattonella verruculosa have been devastating for the farmed salmon industry, but in Southern Chile the conditions that promote the growth and toxicity of these microalgae are poorly understood. This study examined the effects of different combinations of temperature (12, 15, 18 °C) and salinity (10, 20, 30 psu) on the growth of Chilean strains of these two species. The results showed that the optimal growth conditions for H. akashiwo and P. verruculosa differed, with a maximum rate of 0.99 day−1 obtained at 15 °C and a salinity of 20 psu for H. akashiwo, and a maximum rate of 1.06 day−1 obtained at 18 °C and a salinity of 30 psu for P. verruculosa. Cytotoxic assays (2 × 101 – 2 × 105 cell mL−1; cells, filtrates, and cell lysates) performed at salinities of 20 and 30 psu showed a 100% reduction in the viability of embryonic fish cells exposed to intact cells of H. akashiwo and a 39% reduction following exposure to culture filtrates of P. verruculosa. Differences in the fish-killing mechanisms (direct cell contact vs. extracellular substances) and physiological traits of H. akashiwo and P. verruculosa explain the recent occurrence of very large blooms under contrasting (cold-brackish vs. hot-salty) extreme climate conditions in Chile This study was funded by project REDI170575 (Patricio A. Díaz) from the International Cooperation Programme of the National Agency for Research and Development (ANID), Chile. Dr. Carlos Molinet was funded by the FONDAP Project N° 15110027 (INCAR) |
| Databáze: | OpenAIRE |
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