Transcriptome analysis of Corynebacterium pseudotuberculosis biovar Equi in two conditions of the environmental stress
| Autor: | Jorianne Thyeska Castro Alves, Edson Luiz Folador, Thiago Luiz de Paula Castro, Izabela Coimbra Ibraim, Pablo H. C. G. de Sá, Adriana Ribeiro Carneiro Folador, Wanderson M. Silva, Felipe L. Pereira, Fernanda Alves Dorella, Carlos Augusto Gomes Leal, Yuri Rafael de Oliveira Silva, Anne Cybelle Pinto Gomide, Vasco Azevedo, Diego Mariano, Alex F. Carvalho, Artur Silva, Silvanira Barbosa, Mariana Passos Santana, Henrique César Pereira Figueiredo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
STRESS Virulence Factors Corynebacterium pseudotuberculosis Biovar 030106 microbiology Virulence RNA-Seq Biology Genome Ciencias Biológicas Transcriptome 03 medical and health sciences Biología Celular Microbiología Bacterial Proteins Stress Physiological Genetics RT-QPCR Animals TRANSCRIPTOMICS RNA-SEQ Gene BIOVAR EQUI Sheep Corynebacterium Infections Gene Expression Profiling CORYNEBACTERIUM PSEUDOTUBERCULOSIS General Medicine biology.organism_classification Pathogenicity island CIENCIAS NATURALES Y EXACTAS Genome Bacterial |
| Zdroj: | Gene. 677 |
| ISSN: | 1879-0038 |
| Popis: | Corynebacterium pseudotuberculosis has been widely studied in an effort to understand its biological evolution. Transcriptomics has revealed possible candidates for virulence and pathogenicity factors of strain 1002 (biovar Ovis). Because C. pseudotuberculosis is classified into two biovars, Ovis and Equi, it was interesting to assess the transcriptional profile of biovar Equi strain 258, the causative agent of ulcerative lymphangitis. The genome of this strain was re-sequenced; the reassembly was completed using optical mapping technology, and the sequence was subsequently re-annotated. Two growth conditions that occur during the host infection process were simulated for the transcriptome: the osmotic and acid medium. Genes that may be associated with the microorganism's resilience under unfavorable conditions were identified through RNAseq, including genes present in pathogenicity islands. The RT-qPCR was performed to confirm the results in biological triplicate for each condition for some genes. The results extend our knowledge of the factors associated with the spread and persistence of C. pseudotuberculosis during the infection process and suggest possible avenues for studies related to the development of vaccines, diagnosis, and therapies that might help minimize damage to agribusinesses. Fil: Gomide, Anne Cybelle Pinto. Universidade Federal de Minas Gerais; Brasil Fil: Ibraim, Izabela Coimbra. Universidade Federal de Minas Gerais; Brasil Fil: Alves, Jorianne T. C.. Universidade Federal do Pará; Brasil Fil: de Sá, Pablo Gomes. Federal Rural University Of Amazonia; Brasil Fil: de Oliveira Silva, Yuri Rafael. Universidade Federal do Pará; Brasil Fil: Santana, Mariana Passos. Universidade Federal de Minas Gerais; Brasil Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Folador, Edson Luiz. Universidade Federal Da Paraiba; Brasil Fil: Mariano, Diego C. B.. Universidade Federal de Minas Gerais; Brasil Fil: de Paula Castro, Thiago Luiz. Universidade Federal da Bahia; Brasil Fil: Barbosa, Silvanira. Universidade Federal do Pará; Brasil Fil: Dorella, Fernanda Alves. Universidade Federal de Minas Gerais; Brasil Fil: Carvalho, Alex F.. Universidade Federal de Minas Gerais; Brasil Fil: Pereira, Felipe L.. Universidade Federal de Minas Gerais; Brasil Fil: Leal, Carlos A. G.. Universidade Federal de Minas Gerais; Brasil Fil: Figueiredo, Henrique C. P.. Universidade Federal de Minas Gerais; Brasil Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais; Brasil Fil: Silva, Artur. Universidade Federal do Pará; Brasil Fil: Folador, Adriana Ribeiro Carneiro. Universidade Federal do Pará; Brasil |
| Databáze: | OpenAIRE |
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