Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis

Autor: Goran Miljuš, Marko Baralić, Dragana Robajac, Voin Brkovic, Lucia Pažitná, Mirjana Lausevic, Zorana Dobrijević, Ana Penezić, Jaroslav Katrlík, Olgica Nedić, Miloš Šunderić, Nikola Gligorijević
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Biomolecules
Biomolecules, Vol 10, Iss 1165, p 1165 (2020)
Volume 10
Issue 8
ISSN: 2218-273X
Popis: Glycosylation may strongly affect protein structure and functions. A high risk of cardiovascular complications seen in patients with end-stage renal disease (ESRD) is, at least partly associated with delayed clot formation, increased clot strength, and delayed cloth lysis. Taking into consideration that fibrinogen mediates these processes, we isolated fibrinogen from the plasma from patients with ESRD on peritoneal dialysis (ESRD-PD), and examined glycosylation of native fibrinogen and its subunits by lectin-based microarray and lectin blotting. Compared to healthy controls, fibrinogen from patients had increased levels of A2BG2 and decreased levels of FA2 glycan. The distribution of glycans on individual chains was also affected, with the &gamma
chain, responsible for physiological functions of fibrinogen (such as coagulation and platelet aggregation), being most prone to these alterations. Increased levels of multi-antennary N-glycans in ESRD-PD patients were also associated with the type of dialysis solutions, whereas an increase in the fucosylation levels was strongly related to the peritoneal membrane damage. Consequently, investigation of fibrinogen glycans can offer better insight into fibrinogen-related complications observed in ESRD-PD patients and, additionally, contribute to prognosis, choice of personalised therapy, determination of peritoneal membrane damage, and the length of utilization of peritoneum for dialysis.
Databáze: OpenAIRE
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