The closed eye harbours a unique microbiome in dry eye disease
Autor: | Gabriel Rezonzew, Charitharth Vivek Lal, Amelia Freeman, Kelly K. Nichols, Cameron K Postnikoff, Amit Gaggar, Kent A. Willis |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Adult Male Future studies genetic structures medicine.medical_treatment lcsh:Medicine Inflammation Disease Article Microbial ecology Machine Learning 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S medicine Humans Microbiome Ocular disease lcsh:Science Saline Eye diseases Corneal epithelium Multidisciplinary Trauma Severity Indices business.industry Microbiota lcsh:R Middle Aged eye diseases 030104 developmental biology medicine.anatomical_structure Case-Control Studies Tears Immunology 030221 ophthalmology & optometry lcsh:Q Dry Eye Syndromes Female sense organs Metagenomics medicine.symptom business |
Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
ISSN: | 2045-2322 |
Popis: | Dry eye affects millions of individuals. In experimental models, dry eye disease is associated with T helper cell 17-mediated inflammation of the ocular surface that may cause persistent damage to the corneal epithelium. However, the initiating and perpetuating factors associated with chronic inflammation of the ocular surface remain unclear. The ocular microbiota alters ocular surface inflammation and may influence dry eye disease development and progression. Here, we collected serial samples of tears on awakening from sleep, closed eye tears, during a randomized clinical trial of a non-pharmaceutical dry eye therapy and used 16S rRNA metabarcoding to characterize the microbiome. We show the closed dry eye microbiome is distinct from the healthy closed eye microbiome, and that the microbiome remains distinct despite daily saline eye wash upon awakening. The ocular microbiome was described only recently, and this report implicates a distinct microbiome in ocular disease development. Our findings suggest an interplay between microbial commensals and inflammation on the ocular surface. This information may inform future studies of the pathophysiological mechanisms of dry eye disease. |
Databáze: | OpenAIRE |
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