Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning

Autor: Olivier Ouari, Diane Cala de Paepe, Inara Akopjana, Svetlana Kotelovica, G. Pintacuda, Anne Lesage, Andrea Pica, Andrew J. Pell, Kristaps Jaudzems, Andrea Bertarello, Sachin Rama Chaudhari, Emeline Barbet-Massin, Kaspars Tars, David Gajan
Přispěvatelé: Biological Solid-State NMR Methods - Méthodes de RMN à l'état solide en biologie, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Solid-State NMR Methods for Materials - Méthodes de RMN à l'état solide pour les matériaux, Dipartimento di Scienze Chimiche, Università degli studi di Napoli Federico II, Department of Materials and Environmental Chemistry - Arrhenius Laboratory, Biomed Res & Study Ctr, Institut de Chimie Radicalaire (ICR), Aix Marseille Université (AMU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), We acknowledge financial support from the CNRS, the EQUIPEX (contract ANR-10-EQPX-47-01), the Agence Nationale de la Recherche (contract ANR15-CE29-002202), and the People Programme of the European Union's FP7 (FP7- PEOPLE-2012-ITN no. 317127 'pNMR'). K.J. is supported by a MC incoming fellowship (REA grant agreement no. 661175 'virus-DNP-NMR'). A.P. is supported by Fondazione con il Sud (grant 2011-PDR-19)., ANR-10-EQPX-0047,SENS,RMN de Surface Exalté par Polarisation Dynamique Nucléaire(2010), European Project: 317127,EC:FP7:PEOPLE,FP7-PEOPLE-2012-ITN,PNMR(2013), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University of Naples Federico II = Università degli studi di Napoli Federico II
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩
Angewandte Chemie International Edition, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩
ISSN: 1433-7851
1521-3773
DOI: 10.1002/anie.201801016⟩
Popis: International audience; Dynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivity limitation of magic-angle-spinning (MAS) NMR experiments. However, the resolution of the DNPNMR spectra of proteins is compromised by severe line broadening associated with the necessity to perform experiments at cryogenic temperatures and in the presence of paramagnetic radicals. High-quality DNP-enhanced NMR spectra of the Acinetobacter phage 205 (AP205) nucleocapsid can be obtained by combining high magnetic field (800MHz) and fast MAS (40kHz). These conditions yield enhanced resolution and long coherence lifetimes allowing the acquisition of resolved 2D correlation spectra and of previously unfeasible scalar-based experiments. This enables the assignment of aromatic resonances of the AP205 coat protein and its packaged RNA, as well as the detection of long-range contacts, which are not observed at room temperature, opening new possibilities for structure determination.
Databáze: OpenAIRE
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