RNA Replication and Membrane Modification Require the Same Functions of Alphavirus Nonstructural Proteins
| Autor: | Kirsi Hellström, Eija Jokitalo, Katri Kallio, Tero Ahola |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
RNA capping viruses 030106 microbiology Immunology RNA-dependent RNA polymerase Alphavirus Viral Nonstructural Proteins Virus Replication Semliki Forest virus Microbiology 03 medical and health sciences Capping enzyme Virology Polymerase biology Cell Membrane RNA biology.organism_classification Semliki forest virus Molecular biology Genome Replication and Regulation of Viral Gene Expression Cell biology 030104 developmental biology Viral replication Insect Science biology.protein RNA Viral |
| Zdroj: | Journal of Virology. 90:1687-1692 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.02484-15 |
| Popis: | The alphaviruses induce membrane invaginations known as spherules as their RNA replication sites. Here, we show that inactivation of any function (polymerase, helicase, protease, or membrane association) essential for RNA synthesis also prevents the generation of spherule structures in a Semliki Forest virus trans -replication system. Mutants capable of negative-strand synthesis, including those defective in RNA capping, gave rise to spherules. Recruitment of RNA to membranes in the absence of spherule formation was not detected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|