Lactobacillus paracasei feeding improves the control of secondary experimental meningococcal infection in flu-infected mice

Autor: Nouria Belkacem, Raphaëlle Bourdet-Sicard, Muhamed-Kkeir Taha
Přispěvatelé: Infections Bactériennes Invasives, Institut Pasteur [Paris], BIOASTER Microbiology Technology Institute [Lyon], Danone Research, Groupe DANONE, The study was supported by the grants CI IMMUNOBIOTIC 1307014/00 IRT., BAP301 and Danone 30000221, as well as the Institut Pasteur. Danone Nutricia Research provided the Lactobacilli strains and performed the microbiota analysis. The funders had no role in study design, the decision to publish, or preparation of the manuscript., Institut Pasteur [Paris] (IP)
Rok vydání: 2018
Předmět:
0301 basic medicine
Influenzae
Administration
Oral
Neisseria meningitidis
MESH: Meningococcal Infections
medicine.disease_cause
Mice
Medical microbiology
Influenza A virus
Medicine
MESH: Animals
MESH: Orthomyxoviridae Infections
Mice
Inbred BALB C
MESH: Cytokines
biology
Coinfection
Optical Imaging
food and beverages
Lacticaseibacillus paracasei
3. Good health
Infectious Diseases
MESH: Administration
Oral
Cytokines
Female
Research Article
Secondary infection
medicine.medical_specialty
Lactobacillus paracasei
MESH: Lactobacillus paracasei
030106 microbiology
MESH: Mice
Inbred BALB C
MESH: Influenza A Virus
H3N2 Subtype
MESH: Neisseria meningitidis
Virus
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Orthomyxoviridae Infections
Meningococci
Animals
lcsh:RC109-216
MESH: Mice
Inflammation
MESH: Optical Imaging
business.industry
Influenza A Virus
H3N2 Subtype
Probiotics
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Coinfection
Meningococcal Infections
Disease Models
Animal
Parasitology
Nasal administration
MESH: Disease Models
Animal
business
MESH: Female
MESH: Probiotics
Zdroj: BMC Infectious Diseases
BMC Infectious Diseases, BioMed Central, 2018, 18 (1), pp.167. ⟨10.1186/s12879-018-3086-9⟩
BMC Infectious Diseases, Vol 18, Iss 1, Pp 1-9 (2018)
BMC Infectious Diseases, 2018, 18 (1), pp.167. ⟨10.1186/s12879-018-3086-9⟩
ISSN: 1471-2334
DOI: 10.1186/s12879-018-3086-9
Popis: Background The use of probiotics to improve anti-microbial defence, such as for influenza infections, is increasingly recommended. However, no data are available on the effect of probiotics on flu-associated secondary bacterial infections. There is strong evidence of a spatiotemporal association between influenza virus infection and invasive Neisseria meningitidis. We thus investigated the effect of feeding mice Lactobacillus paracasei CNCM I-1518 in a mouse model of sequential influenza-meningococcal infection. Methods We intranasally infected BALB/c mice with a strain of influenza A virus (IAV) H3N2 that was first adapted to mice. Seven days later, a secondary bacterial infection was induced by intranasal administration of bioluminescent N. meningitidis. During the experiment, mice orally received either L. paracasei CNCM I-1518 or PBS as a control. The effect of L. paracasei administration on secondary bacterial infection by N. meningitidis was evaluated. Results Oral consumption of L. paracasei CNCM I-1518 reduced the weight loss of infected mice and lowered the bioluminescent signal of infecting meningococci. This improvement was associated with higher recruitment of inflammatory myeloid cells, such as interstitial monocytes and dendritic cells, to the lungs. Conclusions Our data highlight the role of the gut-lung axis. L. paracasei CNCM I-1518 may boost the defence against IAV infection and secondary bacterial infection, which should be further studied and validated in clinical trials. Electronic supplementary material The online version of this article (10.1186/s12879-018-3086-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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