Evaluation of p16INK4a, minichromosome maintenance protein 2, DNA topoisomerase IIα, ProEX C, and p16INK4a/ProEX C in cervical squamous intraepithelial lesions
| Autor: | William Dupree, Haiyan Liu, Steven C. Meschter, Conrad Schuerch, Yajue Huang, Myra L. Wilkerson, Jianhui Shi, Fan Lin |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Uterine Cervical Neoplasms Cell Cycle Proteins Cervical biopsy Pathology and Forensic Medicine Minichromosome maintenance Minichromosome Antigens Neoplasm Predictive Value of Tests Biopsy Biomarkers Tumor medicine Humans Cyclin-Dependent Kinase Inhibitor p16 Retrospective Studies biology medicine.diagnostic_test Topoisomerase Nuclear Proteins Minichromosome Maintenance Complex Component 2 Anatomical pathology medicine.disease Immunohistochemistry Staining DNA-Binding Proteins Squamous intraepithelial lesion DNA Topoisomerases Type II Carcinoma Squamous Cell biology.protein Female |
| Zdroj: | Human Pathology. 38:1335-1344 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/j.humpath.2007.01.025 |
| Popis: | Summary p16INK4a has been shown to be overexpressed in nearly all high-grade squamous intraepithelial lesions (HSILs). Other cell-cycle regulators, such as minichromosome maintenance protein 2 (MCM2), DNA topoisomerase IIα (TOP IIA), and ProE X C (a cocktail of MCM2 and TOP IIA), have also demonstrated some value in identifying squamous intraepithelial lesions. Data on direct comparison of those cell regulatory proteins in the detection of squamous intraepithelial lesions, with a focus on low-grade squamous intraepithelial lesions (LSILs), are limited. We immunohistochemically evaluated the diagnostic value of p16, MCM2, TOP IIA, ProE X C, and a cocktail of p16 and ProE X C in 62 cervical biopsy specimens, including 14 cases of benign squamous mucosa (group 1), 34 cases of LSILs (group 2), and 14 cases of HSILs (group 3). The staining intensity and distribution were recorded. The results demonstrated that positive staining for p16 and the p16/ProE X C was observed in 100% of cases in group 3, whereas 79%, 86%, and 79% of cases were positive for CM2, TOP IIA, and ProE X C, respectively. ProE X C and the p16/ProE X C showed positive staining in 94% and 100% of cases in group 2, respectively. In contrast, immunoreactivity for p16, MCM2, and TOP IIA was detected in only 76% of cases in group 2. Importantly, all 8 p16-negative cases in group 2 were positive for p16/ProE X C ( P = .003). Our data indicate that (1) p16 is a more sensitive and specific marker for identifying HSILs; (2) ProE X C is a better marker for the detection of LSILs; and (3) p16/ProE X C provides the highest diagnostic value for the detection of both HSILs and LSILs. |
| Databáze: | OpenAIRE |
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