The effects of low-intensity laser therapy on hepatic ischemia-reperfusion injury in a rat model
| Autor: | Mohammad Ashrafzadeh Takhtfooladi, Hamed Ashrafzadeh Takhtfooladi, Mohammadreza Khansari |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pathology Time Factors Urology Dermatology Lipid peroxidation chemistry.chemical_compound Malondialdehyde medicine Animals Aspartate Aminotransferases Low-Level Light Therapy Rats Wistar Liver injury business.industry Tumor Necrosis Factor-alpha Alanine Transaminase Glutathione medicine.disease Rats Transplantation chemistry Liver Reperfusion Injury Surgery Tumor necrosis factor alpha Histopathology Lipid Peroxidation business Reperfusion injury |
| Zdroj: | Lasers in medical science. 29(6) |
| ISSN: | 1435-604X |
| Popis: | Ischemia-reperfusion (I/R) is a major mechanism of liver injury following hepatic surgery or transplantation. Despite numerous reports on the role and relics of low-intensity laser therapy (LILT) in many organs, the potential effects of LILT on hepatic ischemia-reperfusion have not been explored. This study was aimed to investigate the impresses of LILT applied to the skin following hepatic ischemia and reperfusion. Thirty-six healthy male Wistar rats were allocated into three groups of twelve animals each as follows: Sham, Ischemia-reperfusion (IR), and Ischemia-reperfusion with laser treatment (IR+LILT). Hepatic ischemia was induced by clamping the arterial and portal venous for 45 min. A laser diode (400 mW, 804 nm) was applied to the skin surface at the anatomical site of the liver at a dose of 3 J/cm(2), and the duration of irradiation was selected 120 s with 15-min interval after beginning the reperfusion. Animals were maintained under anesthesia and sacrificed 6 h subsequent reperfusion. Hepatic samples were evaluated for histological assessment and biochemistry analysis. Serum aminotransferase levels, tumor necrosis factor-alpha (TNF-α) levels, malondialdehyde (MDA), and glutathione (GSH) levels were significantly lower (P 0.05) in the irradiated group compared to the I/R group during the 6 h after reperfusion. The number of histopathological changes in the hepatic tissues was significantly lower in the treated group (P 0.05). These observations suggest that LILT applied in transcutaneous manner effectively improves hepatic injuries after ischemia-reperfusion period in rats. |
| Databáze: | OpenAIRE |
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