Direct Detection of Wild Poliovirus Circulation by Stool Surveys of Healthy Children and Analysis of Community Wastewater
| Autor: | C. A. de Quadros, J Boshell, Gina Tambini, Elizabeth Andrade Marques, Olen M. Kew, Jon Kim Andrus, Mark A. Pallansch |
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| Rok vydání: | 1993 |
| Předmět: |
Male
viruses Sewage Colombia medicine.disease_cause Polymerase Chain Reaction complex mixtures law.invention Feces law Genotype medicine Humans Immunology and Allergy Polymerase chain reaction business.industry Poliovirus Infant Newborn Wild type Infant medicine.disease Virology Poliomyelitis Infectious Diseases Child Preschool Enterovirus Female Water Microbiology business |
| Zdroj: | Journal of Infectious Diseases. 168:1510-1514 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/168.6.1510 |
| Popis: | Cartagena, Colombia, was one of the last cities in the Americas known to have endemic poliomyelitis. After 3 cases were identified in 1991, two approaches for detecting continued silent transmission of wild polioviruses within a high-risk community were used: stool surveys of healthy children and virologic analysis of community sewage. Wild type 1 polioviruses were isolated from 8% of the children studied and from 21% of sewage samples. The proportions of wild polioviruses, vaccine-related polioviruses, and nonpolio enteric viruses were similar for both approaches. Wild poliovirus sequences were also amplified directly from processed sewage samples by the polymerase chain reaction using primer pairs specific for the indigenous type 1 genotype. The last reported cases associated with wild polioviruses in the Americas occurred in Colombia (8 April 1991) and Peru (23 August 1991). Direct sampling for wild polioviruses in high-risk communities can provide further evidence that eradication of the indigenous wild polioviruses has been achieved in the Americas. |
| Databáze: | OpenAIRE |
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