Description of Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ANI calculations
| Autor: | Ana Cifuentes, Ramon Rosselló-Móra, Peter Kämpfer, Aránzazu López-López, Daniel Ramón, Guillermo Jiménez, Javier Tamames, Juan F. Martínez, Mercedes Urdiain, Anne-Brit Kolstø, Anicet R. Blanch, Francisco M. Codoñer |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Bacillus cereus Bacillus Applied Microbiology and Biotechnology Microbiology Genome DNA Ribosomal law.invention Probiotic Japan Phylogenetics law Group (periodic table) RNA Ribosomal 16S Animals Cluster Analysis Organic Chemicals Ecology Evolution Behavior and Systematics Phylogeny Bacillus (shape) biology Strain (biology) Probiotics Sequence Analysis DNA biology.organism_classification Animal Feed Bacterial Typing Techniques Cereus Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Dietary Supplements Genome Bacterial |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0723-2020 |
| Popis: | Jiménez, Guillermo et al. Strain BCT-7112T was isolated in 1966 in Japan from a survey designed to obtain naturally occurring microorganisms as pure cultures in the laboratory for use as probiotics in animal nutrition. This strain, which was primarily identified as Bacillus cereus var toyoi, has been in use for more than 30 years as the active ingredient of the preparation TOYOCERIN®, an additive for use in animal nutrition (e.g. swine, poultry, cattle, rabbits and aquaculture). Despite the fact that the strain was initially classified as B. cereus, it showed significant genomic differences from the type strains of the B. cereus group that were large enough (ANI values below 92%) to allow it to be considered as a different species within the group. The polyphasic taxonomic study presented here provides sufficient discriminative parameters to classify BCT-7112T as a new species for which the name Bacillus toyonensis sp. nov. is proposed, with BCT-7112T (=CECT 876T; =NCIMB 14858T) being designated as the type strain. In addition, a pairwise comparison between the available genomes of the whole B. cereus group by means of average nucleotide identity (ANI) calculations indicated that besides the eight classified species (including B. toyonensis), additional genomospecies could be detected, and most of them also had ANI values below 94%. ANI values were on the borderline of a species definition only in the cases of representatives of B. cereus versus B. thuringiensis, and B. mycoides and B. weihenstephanensis. © 2013 Elsevier GmbH. |
| Databáze: | OpenAIRE |
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