Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
| Autor: | Kyuhyong Park, Kook Sun Ha, Wooyoung Kang, Heesoo Uhm, Ja Yil Lee, Changwon Kang, Sungchul Hohng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
DNA Bacterial Transcription Genetic Science General Physics and Astronomy Sigma Factor General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Single-molecule biophysics Sigma factor Transcription (biology) RNA polymerase Intrinsic termination Escherichia coli lcsh:Science Promoter Regions Genetic Polymerase Transcription Initiation Genetic Fluorescent Dyes Terminator Regions Genetic Multidisciplinary biology Escherichia coli Proteins RNA Promoter General Chemistry DNA-Directed RNA Polymerases Carbocyanines Single Molecule Imaging Cell biology 030104 developmental biology Terminator (genetics) chemistry Transcription Termination Genetic Transcription preinitiation complex biology.protein bacteria lcsh:Q Transcription 030217 neurology & neurosurgery DNA |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time. Bacterial transcription is terminated when RNA polymerases encounter terminator sequences. Using a single-molecule fluorescence assay, here the authors show that the release of transcript RNA precedes RNA polymerase dissociation and that the remaining RNA polymerase can reinitiate at nearby promoters. |
| Databáze: | OpenAIRE |
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