Comparison of Cryoprotective Effects of Lycopene and Cysteamine in Different Cryoprotectants on Bull Semen and Fertility Results
| Autor: | Erdem Coskun, Emre Durmaz, Umut Taşdemir, Ayşe Eken, Olga Büyükleblebici, Serhat Büyükleblebici, Pürhan Barbaros Tuncer |
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| Přispěvatelé: | Veteriner Fakültesi, Durmaz, Emre -- 0000-0003-2239-1762, TUNCER, Purhan Barbaros -- 0000-0002-9257-9544 |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Glycerol
Male Tris Ethylene Glycol Cryoprotectant Cysteamine Fertilization in Vitro law.invention chemistry.chemical_compound Cryoprotective Agents Lycopene Endocrinology law Animals Food science Sperm motility Cryopreservation Extender Carotenoids Spermatozoa Semen Analysis chemistry Biochemistry Sperm Motility Cattle Animal Science and Zoology Ethylene glycol Semen Preservation Biotechnology |
| Popis: | WOS: 000342132400011 PubMed: 24942070 Contents The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty-four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5mm or lycopene 500g/ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol with lycopene resulted in a better rate of CASA progressive motility compared with that of other groups. Generally, all the lycopene groups signed better protective effects on acrosome and total morphology than the other groups. Glycerol 7% and 3% ethylene glycol with lycopene groups yielded to slight higher percentages of membrane integrity assessed by HOST than that of the other groups, but 7% glycerol with cysteamine and 3% ethylene glycol with cysteamine showed the worst percentages of membrane integrity. Glycerol 7% and 5% glycerol with lycopene gave rise to a higher value of VAP, VSL and VCL compared with that of the other groups. On the contrary, adding to 5% glycerol with cysteamine showed negative effect for VAP, VSL, VCL and ALH values. All cryoprotectant groups with lycopene decreased chromatin damage than the other groups. Ethylene glycol 3% led to lower non-return rates of inseminated cows. However, this result was not considered to be statistically important. Republic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) [09/01/01/01] This study was supported by the Republic of Turkey, Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Research (GDAR) Project number: 09/01/01/01. |
| Databáze: | OpenAIRE |
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