Recovery of infectious human immunodeficiency virus from cells treated with 1% paraformaldehyde
| Autor: | Janet K. A. Nicholson, Carol H. Aloisio |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cell Survival
Polymers Lymphocyte T-Lymphocytes Immunology Human immunodeficiency virus (HIV) Biology medicine.disease_cause Virus Microbiology Cell Line chemistry.chemical_compound Antigen Antigens CD Formaldehyde medicine Immunology and Allergy Humans Phytohemagglutinins Paraformaldehyde Cells Cultured Temperature Antibodies Monoclonal HIV Virology Titer medicine.anatomical_structure chemistry Cell culture biology.protein Antibody |
| Zdroj: | Journal of immunological methods. 128(2) |
| ISSN: | 0022-1759 |
| Popis: | Fixation and preservation of cells with 1% paraformaldehyde is a common practice in laboratories performing flow cytometric analysis of cells from persons infected with human immunodeficiency virus (HIV). Previous studies indicated that 1% paraformaldehyde effectively inactivates cell-free HIV. In this study, we tested the inactivation capacity of 1% paraformaldehyde on a cell line chronically infected with HIV and human peripheral blood lymphocytes acutely infected with the virus. We found a significant decrease in the infectious titer of the cells after treatment with 1% paraformaldehyde. However, we did not find total inactivation of the specimens treated for up to 18 h. |
| Databáze: | OpenAIRE |
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