Molecular and phylogenetic analyses of the liver amphistome Explanatum explanatum (Creplin, 1847) Fukui, 1929 in ruminants from Bangladesh and Nepal based on nuclear ribosomal ITS2 and mitochondrial nad1 sequences
| Autor: | Tadashi Itagaki, Uday Kumar Mohanta, Hari Bahadur Rana, Bhuminand Devkota |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine Buffaloes Genotype Liver Diseases Parasitic Trematode Infections Biology Fixation index 03 medical and health sciences Nepal DNA Ribosomal Spacer Animals Cluster Analysis Internal transcribed spacer Domestication Ribosomal DNA Phylogeny Bangladesh Phylogenetic tree business.industry NADH Dehydrogenase General Medicine Sequence Analysis DNA 030108 mycology & parasitology Ribosomal RNA DNA Helminth 030104 developmental biology Animal Science and Zoology Parasitology Livestock Cattle Trematoda business |
| Zdroj: | Journal of helminthology. 91(4) |
| ISSN: | 1475-2697 |
| Popis: | Explanatum explanatum flukes, liver amphistomes of ruminants, cause significant economic loss in the livestock industry by inducing severe liver damage. A total of 66 flukes from 26 buffaloes and 7 cattle in four different geographic areas of Bangladesh and 20 flukes from 10 buffaloes in the Chitwan district of Nepal were subjected for analysis. The sequences (442 bp) of the second internal transcribed spacer (ITS2) of ribosomal DNA and the variable fragments (657 bp) of mitochondrial nicotinamide dehydrogenase subunit 1 (nad1) of E. explanatum flukes from Bangladesh and Nepal were analysed. The aim of this study was molecular characterization of the flukes and to elucidate their origin and biogeography. In the ITS2 region, two genotypes were detected among the flukes from Bangladesh, while flukes from Nepal were of only one genotype. Phylogenetic analyses inferred from the nad1 gene revealed that at least four divergent populations (groups I–IV) are distributed in Bangladesh, whereas two divergent populations were found to be distributed in Nepal. Fst values (pairwise fixation index) suggest that Bangladeshi and Nepalese populations of group I to IV are significantly different from each other; but within groups III and IV, the populations from Bangladesh and Nepal were genetically close. This divergence in the nad1 gene indicates that each lineage of E. explanatum from diverse geography was co-adapted during the multiple domestication events of ruminants. This study, for the first time, provides molecular characterization of E. explanatum in Bangladesh and Nepal, and may provide useful information for elucidating its origin and dispersal route in Asia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|