The identification of a nuclear factor Akirin with regulating the expression of antimicrobial peptides in red swamp crayfish (Procambarus clarkii)
| Autor: | Hui Yang, Haoran Xiong, Yingying Zhang, Yinan Jiang, Wenzhi Wei, Tongwei Ji |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Antimicrobial peptides Astacoidea 02 engineering and technology Biochemistry Arthropod Proteins Microbiology 03 medical and health sciences White spot syndrome virus 1 Immune system Structural Biology RNA interference Animals Tissue Distribution Amino Acid Sequence Cloning Molecular Molecular Biology Gene 030304 developmental biology Procambarus clarkii 0303 health sciences Innate immune system biology Gene Expression Profiling Nuclear Proteins General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Crayfish Immunity Innate Gene Expression Regulation Hepatopancreas 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 183:707-717 |
| ISSN: | 0141-8130 |
| Popis: | Akirin is a highly conserved nuclear factor among different species. It is closely related to skeletal muscle development, innate immune response, and tumorigenesis in a variety of animals. In invertebrates, Akirin is mainly involved in gene transcription and NF-κB dependent natural immune response. In the present study, a nuclear factor Akirin was identified from Procambarus clarkii. The Akirin protein of crayfish consists of 204 amino acids and is conserved among its family members, especially the nuclear localization signal peptide motif (KRRR). PcAkirin was highly expressed in stomach, intestines, and hepatopancreas. After A. hydrophila challenge, the transcription level of Akirin significantly increased in hemocyte and hepatopancreas. In addition, the recombinant Akirin protein was produced successfully and helpful to resist WSSV infection by increasing the expression level of some immune related genes. On the contrary, after interfering with Akirin gene by dsRNA, the crayfish increased the sensitivity to A. hydrophila and WSSV infections. The results are more obvious in the accumulated mortality of P. clarkii infected with A. hydrophila and WSSV. All these results suggested that Akirin played a significant role in innate immune responses and protected it from WSSV and bacterial infection in crayfish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect