HIV vaccine candidates generate in vitro T cell response to putative epitopes in Chinese-origin rhesus macaques
| Autor: | Ying Liu, Yiming Shao, Peng Meng, Yichen Lu, Zhongfang Zhao, Yanhou Liu, Amie J. Strong, Qi Liao, Neal Touzjian, Yang Fu |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Enzyme-Linked Immunospot Assay
Recombinant Fusion Proteins T-Lymphocytes HIV Core Protein p24 HIV Infections Human leukocyte antigen Major histocompatibility complex Epitope Epitopes Immune system Peptide Library Vaccines DNA Animals HIV vaccine AIDS Vaccines General Veterinary General Immunology and Microbiology biology ELISPOT Public Health Environmental and Occupational Health env Gene Products Human Immunodeficiency Virus biology.organism_classification Fusion protein Virology Macaca mulatta Rhesus macaque Disease Models Animal Infectious Diseases HLA-B Antigens Immunology biology.protein Molecular Medicine |
| Zdroj: | Vaccine. 30(9) |
| ISSN: | 1873-2518 |
| Popis: | The Indian rhesus macaque is the established animal model for HIV infection and vaccine research. Growing evidence suggests that the more readily available Chinese rhesus macaque may be a more relevant option. As increasing numbers of novel Chinese rhesus MHC alleles are reported, we decided to explore potential HIV vaccine epitopes in this model. We immunized forty Chinese rhesus macaques with three different HIV vaccine candidates either individually or following a prime/boost strategy. We used ELISPOT to measure immune response in vitro to HIV-1 p24C and HIV-1 gp160 peptide libraries. We identified five putative epitopes with associations to HLA-I alleles including HLA*B-2705 and HLA-B*5101 (associated with slow disease progression and low viral set point) and HLA-B*18 (associated with rapid disease progression and high viral set point). This suggests the possible use of Chinese rhesus macaques to model different disease progressions. We also explored the use of fusion proteins as stimulators in ELISPOT assays. While PBMCs from 6 monkeys responded to peptide stimulation, PBMCs from 28 monkeys responded to the anthrax lethal factor fusion proteins LFn p24C and/or LFn gp140C. Our results support the use of Chinese rhesus macaques in HIV vaccine studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect