A murine antibody to Shigella dysenteriae type 1 employs V-genes that contain a rearranged codon for the λ light chain
| ISSN: | 0161-5890 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cbcbe6829833e9b06f0b89eae5696a1 https://doi.org/10.1016/0161-5890(95)00072-m |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....1cbcbe6829833e9b06f0b89eae5696a1 |
| Autor: | D. Siwarski, Cornelis P.J. Glaudemans, Charles E. Miller, Konrad Huppi, Arthur B. Karpas, C R Mainhart, Andrew Newman |
| Rok vydání: | 1995 |
| Předmět: |
Shigella dysenteriae
Molecular Sequence Data Immunology Immunoglobulin Variable Region Immunoglobulin light chain Mice Immunoglobulin lambda-Chains Antigen Complementary DNA Animals Point Mutation Amino Acid Sequence Codon Molecular Biology Peptide sequence Gene DNA Primers Genetics Hybridomas Base Sequence Genes Immunoglobulin biology Bacterial polysaccharide Antibodies Monoclonal biology.organism_classification Antibodies Bacterial Molecular biology biology.protein Antibody |
| Zdroj: | Molecular Immunology. 32:679-682 |
| ISSN: | 0161-5890 |
| Popis: | The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding antibodies, while the VL is closely related to the V lambda 1 gene. We found that the VL3707 E9 gene employed a VL-J combinatorial joining leading to a rare Trp-->Leu substitution at position L96. |
| Databáze: | OpenAIRE |
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