Select neurotrophins promote oligodendrocyte progenitor cell process outgrowth in the presence of chondroitin sulfate proteoglycans
Autor: | Justin R. Siebert, Donna J. Osterhout |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Ciliary neurotrophic factor Rats Sprague-Dawley Myelin 0302 clinical medicine Neurotrophin 3 Neurotrophic factors Glial cell line-derived neurotrophic factor Neurons brain‐derived neurotrophic factor Cell Death RRID:AB_2535847 RRID:AB_357617 oligodendrocyte progenitor cells Cell Differentiation Cell biology medicine.anatomical_structure neurotrophic factor 3 Female RRID:CVCL_0154 Research Article Neurotrophin glial cell line‐derived neurotrophic factor Primary Cell Culture chondroitin sulfate proteoglycans Biology Glial scar 03 medical and health sciences Cellular and Molecular Neuroscience medicine Animals RRID:AB_1157905 Ciliary Neurotrophic Factor Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Spinal Cord Injuries Cell Proliferation Oligodendrocyte Precursor Cells Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor Regeneration (biology) Nerve Regeneration Rats RRID:RGD_1566440 030104 developmental biology Animals Newborn nervous system biology.protein 030217 neurology & neurosurgery |
Zdroj: | Journal of Neuroscience Research |
ISSN: | 1097-4547 0360-4012 |
DOI: | 10.1002/jnr.24780 |
Popis: | Axonal damage and the subsequent interruption of intact neuronal pathways in the spinal cord are largely responsible for the loss of motor function after injury. Further exacerbating this loss is the demyelination of neighboring uninjured axons. The post‐injury environment is hostile to repair, with inflammation, a high expression of chondroitin sulfate proteoglycans (CSPGs) around the glial scar, and myelin breakdown. Numerous studies have demonstrated that treatment with the enzyme chondroitinase ABC (cABC) creates a permissive environment around a spinal lesion that permits axonal regeneration. Neurotrophic factors like brain‐derived neurotrophic factor (BDNF), glial cell line‐derived neurotrophic factor (GDNF), neurotrophic factor‐3 (NT‐3), and ciliary neurotrophic factor (CNTF) have been used to promote neuronal survival and stimulate axonal growth. CSPGs expressed near a lesion also inhibit migration and differentiation of endogenous oligodendrocyte progenitor cells (OPCs) in the spinal cord, and cABC treatment can neutralize this inhibition. This study examined the neurotrophins commonly used to stimulate axonal regeneration after injury and their potential effects on OPCs cultured in the presence of CSPGs. The results reveal differential effects on OPCs, with BDNF and GDNF promoting process outgrowth and NT‐3 stimulating differentiation of OPCs, while CNTF appears to have no observable effect. This finding suggests that certain neurotrophic agents commonly utilized to stimulate axonal regeneration after a spinal injury may also have a beneficial effect on the endogenous oligodendroglial cells as well. Oligodendrocyte progenitor cells cannot extend cellular process in the presence of Chondroitin Sulfate Proteoglycans. Treatment of OPCs with different neurotrophic factors (BDNF, GDNF, and NT‐3) allows OPCs to overcome the inhibitory effects of CSPGs, allowing for OPC process outgrowth which is a critical step in the differentiation of OPCs. |
Databáze: | OpenAIRE |
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