Select neurotrophins promote oligodendrocyte progenitor cell process outgrowth in the presence of chondroitin sulfate proteoglycans

Autor: Justin R. Siebert, Donna J. Osterhout
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Ciliary neurotrophic factor
Rats
Sprague-Dawley

Myelin
0302 clinical medicine
Neurotrophin 3
Neurotrophic factors
Glial cell line-derived neurotrophic factor
Neurons
brain‐derived neurotrophic factor
Cell Death
RRID:AB_2535847
RRID:AB_357617
oligodendrocyte progenitor cells
Cell Differentiation
Cell biology
medicine.anatomical_structure
neurotrophic factor 3
Female
RRID:CVCL_0154
Research Article
Neurotrophin
glial cell line‐derived neurotrophic factor
Primary Cell Culture
chondroitin sulfate proteoglycans
Biology
Glial scar
03 medical and health sciences
Cellular and Molecular Neuroscience
medicine
Animals
RRID:AB_1157905
Ciliary Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
Spinal Cord Injuries
Cell Proliferation
Oligodendrocyte Precursor Cells
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor
Regeneration (biology)
Nerve Regeneration
Rats
RRID:RGD_1566440
030104 developmental biology
Animals
Newborn

nervous system
biology.protein
030217 neurology & neurosurgery
Zdroj: Journal of Neuroscience Research
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.24780
Popis: Axonal damage and the subsequent interruption of intact neuronal pathways in the spinal cord are largely responsible for the loss of motor function after injury. Further exacerbating this loss is the demyelination of neighboring uninjured axons. The post‐injury environment is hostile to repair, with inflammation, a high expression of chondroitin sulfate proteoglycans (CSPGs) around the glial scar, and myelin breakdown. Numerous studies have demonstrated that treatment with the enzyme chondroitinase ABC (cABC) creates a permissive environment around a spinal lesion that permits axonal regeneration. Neurotrophic factors like brain‐derived neurotrophic factor (BDNF), glial cell line‐derived neurotrophic factor (GDNF), neurotrophic factor‐3 (NT‐3), and ciliary neurotrophic factor (CNTF) have been used to promote neuronal survival and stimulate axonal growth. CSPGs expressed near a lesion also inhibit migration and differentiation of endogenous oligodendrocyte progenitor cells (OPCs) in the spinal cord, and cABC treatment can neutralize this inhibition. This study examined the neurotrophins commonly used to stimulate axonal regeneration after injury and their potential effects on OPCs cultured in the presence of CSPGs. The results reveal differential effects on OPCs, with BDNF and GDNF promoting process outgrowth and NT‐3 stimulating differentiation of OPCs, while CNTF appears to have no observable effect. This finding suggests that certain neurotrophic agents commonly utilized to stimulate axonal regeneration after a spinal injury may also have a beneficial effect on the endogenous oligodendroglial cells as well.
Oligodendrocyte progenitor cells cannot extend cellular process in the presence of Chondroitin Sulfate Proteoglycans. Treatment of OPCs with different neurotrophic factors (BDNF, GDNF, and NT‐3) allows OPCs to overcome the inhibitory effects of CSPGs, allowing for OPC process outgrowth which is a critical step in the differentiation of OPCs.
Databáze: OpenAIRE