Fast Leaps between Millisecond Confinements Govern Ase1 Diffusion along Microtubules
| Autor: | Verena Henrichs, Zdeněk Lánský, Ivan Barvík, Marek Piliarik, Łukasz Bujak, Marcus Braun, Kristýna Holanová, Antonio García Marín |
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| Rok vydání: | 2021 |
| Předmět: |
Millisecond
Chemistry Swine Energy landscape General Chemistry Molecular Dynamics Simulation Microtubules Single Molecule Imaging Protein Transport Spatio-Temporal Analysis Protein Domains Chemical physics Animals General Materials Science Diffusion (business) Microtubule-Associated Proteins LEAPS Cell Division |
| Zdroj: | Small methods. 5(10) |
| ISSN: | 2366-9608 |
| Popis: | Diffusion is the most fundamental mode of protein translocation within cells. Confined diffusion of proteins along the electrostatic potential constituted by the surface of microtubules, although modeled meticulously in molecular dynamics simulations, has not been experimentally observed in real-time. Here, we used interferometric scattering microscopy to directly visualize the movement of the microtubule-associated protein Ase1 along the microtubule surface at nanometer and microsecond resolution. We resolved millisecond confinements of Ase1 and fast leaps between these positions of dwelling preferentially occurring along the microtubule protofilaments, revealing Ase1’s mode of diffusive translocation along the microtubule’s periodic surface. The derived interaction potential closely matches the tubulin-dimer periodicity and the distribution of the electrostatic potential on the microtubule lattice. We anticipate that mapping the interaction landscapes for different proteins on microtubules, finding plausible energetic barriers of different positioning and heights, will provide valuable insights into regulating the dynamics of essential cytoskeletal processes, such as intracellular cargo trafficking, cell division, and morphogenesis, all of which rely on diffusive translocation of proteins along microtubules. |
| Databáze: | OpenAIRE |
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