Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
| Autor: | Valeria V. Orlova, Francijna E. van den Hil, Christine L. Mummery, Oleh V. Halaidych |
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| Přispěvatelé: | Applied Stem Cell Technology |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
General Chemical Engineering Cell Population Induced Pluripotent Stem Cells Microfluidics Inflammatory responses Issue 141 General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Downregulation and upregulation Live cell imaging Developmental biology medicine Cell Adhesion Leukocytes Humans Receptor education Induced pluripotent stem cell Flow adhesion assay Cells Cultured Live imaging education.field_of_study General Immunology and Microbiology Chemistry General Neuroscience Human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs) Endothelial Cells Cell Differentiation Adhesion Cell biology 030104 developmental biology medicine.anatomical_structure |
| Zdroj: | Journal of visualized experiments, 2018(141):e58678. MYJoVE Corporation Journal of Visualized Experiments Journal of Visualized Experiments. JOURNAL OF VISUALIZED EXPERIMENTS ISSUE=141;TITLE=Journal of Visualized Experiments |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58678-v |
| Popis: | Endothelial cells (ECs) are essential for the regulation of inflammatory responses by either limiting or facilitating leukocyte recruitment into affected tissues via a well-characterized cascade of pro-adhesive receptors which are upregulated on the leukocyte cell surface upon the inflammatory trigger. Inflammatory responses differ between individuals in the population and the genetic background can contribute to these differences. Human induced pluripotent stem cells (hiPSCs) have been shown to be a reliable source of ECs (hiPSC-ECs), thus representing an unlimited source of cells that capture the genetic identity and any genetic variants or mutations of the donor. hiPSC-ECs can therefore be used for modeling inflammatory responses in donor-specific cells. Inflammatory responses can be modeled by determining leukocyte adhesion to the hiPSC-ECs under physiological flow. This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow. |
| Databáze: | OpenAIRE |
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