CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells
| Autor: | Shosuke Imai, Hiroshi Wakiguchi, Atsushi Kato, Hirohisa Saito, Takahisa Ogasawara, Jonathan Batchelor, Toshiki Homma, Kenji Matsumoto |
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| Rok vydání: | 2004 |
| Předmět: |
Chemokine
Receptors CXCR3 CpG Oligodeoxynucleotide p38 mitogen-activated protein kinases Biophysics chemical and pharmacologic phenomena CXCR3 Biochemistry Cell Line Immune system Humans Receptor Molecular Biology B-Lymphocytes biology Dose-Response Relationship Drug Chemistry TLR9 hemic and immune systems Cell Biology Molecular biology Cell biology stomatognathic diseases Oligodeoxyribonucleotides Cell culture biology.protein Receptors Chemokine Chemokines CXC |
| Zdroj: | Biochemical and biophysical research communications. 320(4) |
| ISSN: | 0006-291X |
| Popis: | CpG oligodeoxynucleotides (CpG ODN) are known to elicit Th1 immune responses via TLR9. However, the precise mechanisms through which B cells are involved in this phenomenon are not fully understood. We investigated the effect of CpG ODN on the induction of Th1-chemoattractant CXCR3 chemokines, IP-10, Mig, and I-TAC, in B cells. Cells from the RPMI 8226 human B cell line and human peripheral B cells were stimulated with three distinct classes of CpG ODN. As a result, CXCR3 chemokines were strongly up-regulated by CpG-B and CpG-C, but only weakly by CpG-A. Though CXCR3 chemokines are known to be induced by IFNs, blocking mAbs against IFN receptors did not inhibit their induction by CpG-B. Induction of CXCR3 chemokines was blocked by two NF-kappaB inhibitors and a p38 inhibitor. These results strongly suggest that CXCR3 chemokines are directly induced by CpG ODN via NF-kappaB- and p38-dependent pathways in human B cells. |
| Databáze: | OpenAIRE |
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