Assessment of Cellular Proliferation of Sebaceous Neoplasms by AgNOR Counts and Immunohistochemical Demonstrations of PCNA and Ki-67
| Autor: | Kazuo Aso, Shigeo Kondo, Shin Koseki, Yutaka Hozumi, Shin-ichi Ansai |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Dermatology Biology Sebaceoma Sebaceous Glands Proliferating Cell Nuclear Antigen Nucleolus Organizer Region medicine Humans Basal cell carcinoma Sebaceous Gland Neoplasms Grading (tumors) Cell Nucleus Nuclear Proteins General Medicine medicine.disease Immunohistochemistry Neoplasm Proteins Proliferating cell nuclear antigen Ki-67 Antigen Ki-67 biology.protein Nucleolus organizer region Cell Division Sebaceous carcinoma |
| Zdroj: | The Journal of Dermatology. 22:238-248 |
| ISSN: | 0385-2407 |
| DOI: | 10.1111/j.1346-8138.1995.tb03380.x |
| Popis: | We assessed cellular proliferation of sebaceous neoplasms by AgNOR counts and the immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA) and Ki-67, using formalin-fixed and paraffin-embedded tissue specimens. We used three categories of sebaceous neoplasms: four cases of sebaceoma, three cases of basal cell carcinoma with sebaceous differentiation (BCSD), and seven cases of sebaceous carcinoma (SC). Significant differences were noted between SC and non-SC tumors (sebaceoma and BCSD) in AgNOR counts and semi-quantitative grading of PCNA and Ki-67 labelling indices (P < 0.01). When a cut-off value of 6 was chosen, the AgNOR value discriminated SC from non-SC tumors with high specificity and sensitivity. When a cut-off value of 25% was chosen, PCNA and Ki-67 labelling indices also discriminated between these tumors. Significant differences were not observed between sebaceoma and BCSD with PCNA and Ki-67 labelling indices. AgNOR counts of BCSD were a little higher than those of sebaceoma, but the number of cases was too small to perform statistical assessment. We consider AgNOR counts and semi-quantitative grading of PCNA and Ki-67 labelling indices to be useful in differentiating SC from BCSD and sebaceoma. |
| Databáze: | OpenAIRE |
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