Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang onmast cells and allergic rhinitis

Autor: Young Na Park, Youn Ju Lee, Eunkyung Lee, Kyu-Tae Jeong, Sun-Gun Kim, Keuk-Jun Kim, Na-Young Park, Jiean Lee, Hyo-Hyun Park, Hwadong Lee
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
Anti-Inflammatory Agents
Immunoglobulin E
law.invention
chemistry.chemical_compound
law
Anti-Allergic Agents
Medicine
Mast Cells
Allergic rhinitis (AR)
Angelica
Mice
Inbred BALB C

Mice
Inbred ICR

biology
Traditional medicine
Prostaglandin D2
Degranulation
Interleukin
General Medicine
Medicine
Korean Traditional

Female
Bone-marrow derived mast cells (BMMC)
Mentha
Research Article
Passive cutaneous anaphylaxis (PCA)
Biyeom-Tang
Bone-marrow derived mast cells(BMMC)
Prostaglandin D-2 (PGD(2))
Leukotriene C-4 (LTC4)
Bone Marrow Cells
Trichosanthes
In vivo
Animals
Leukotriene C4
business.industry
Plant Extracts
Interleukins
Xanthium
Rhinitis
Allergic

In vitro
Disease Models
Animal

Complementary and alternative medicine
chemistry
Prostaglandin D2 (PGD2)
biology.protein
business
Phytotherapy
Leukotriene C4 (LTC4)
Zdroj: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(14)
BMC Complementary and Alternative Medicine
Popis: Background Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models. Methods The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo. Results EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced. Conclusions Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.
Databáze: OpenAIRE
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