Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang onmast cells and allergic rhinitis
Autor: | Young Na Park, Youn Ju Lee, Eunkyung Lee, Kyu-Tae Jeong, Sun-Gun Kim, Keuk-Jun Kim, Na-Young Park, Jiean Lee, Hyo-Hyun Park, Hwadong Lee |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Male
Anti-Inflammatory Agents Immunoglobulin E law.invention chemistry.chemical_compound law Anti-Allergic Agents Medicine Mast Cells Allergic rhinitis (AR) Angelica Mice Inbred BALB C Mice Inbred ICR biology Traditional medicine Prostaglandin D2 Degranulation Interleukin General Medicine Medicine Korean Traditional Female Bone-marrow derived mast cells (BMMC) Mentha Research Article Passive cutaneous anaphylaxis (PCA) Biyeom-Tang Bone-marrow derived mast cells(BMMC) Prostaglandin D-2 (PGD(2)) Leukotriene C-4 (LTC4) Bone Marrow Cells Trichosanthes In vivo Animals Leukotriene C4 business.industry Plant Extracts Interleukins Xanthium Rhinitis Allergic In vitro Disease Models Animal Complementary and alternative medicine chemistry Prostaglandin D2 (PGD2) biology.protein business Phytotherapy Leukotriene C4 (LTC4) |
Zdroj: | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(14) BMC Complementary and Alternative Medicine |
Popis: | Background Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models. Methods The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo. Results EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced. Conclusions Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models. |
Databáze: | OpenAIRE |
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