Cell-cell interactions influence survival and differentiation of purified purkinje cells in vitro
| Autor: | Carlos A. Baptista, Mary E. Hatten, Richard Blazeski, Carol A. Mason |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cerebellum
Cell signaling Cell Survival Cellular differentiation Cytological Techniques Cell Purkinje cell Cell Communication Biology Mice Purkinje Cells Nerve Fibers Cell–cell interaction Culture Techniques Pons medicine Animals Afferent Pathways General Neuroscience Granule (cell biology) Cell Differentiation In vitro Cell biology Mice Inbred C57BL medicine.anatomical_structure Astrocytes Neuroscience Granulocytes |
| Zdroj: | Neuron. 12:243-260 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/0896-6273(94)90268-2 |
| Popis: | To determine the role of cell-cell interactions in Purkinje cell survival and dendritic differentiation, perinatal mouse Purkinje cells were purified, and their development was analyzed in vitro. In isolation at low density, Purkinje cell survival was poor, improved by neuronal contacts, either with purified granule neurons or with Purkinje cells themselves. Moreover, coculture with specific cell populations led to widely different degrees of Purkinje cell differentiation. Purified Purkinje cells cultured alone or with an inappropriate afferent, the mossy fibers, did not progress beyond immature forms. With astroglia, Purkinje cells had thin smooth processes. Proper Purkinje cell differentiation was driven only by coculture with granule cells, resulting in dendrites with spines receiving synapses. These results suggest that Purkinje cell differentiation is regulated by local epigenetic factors, provided in large part by the granule neuron. |
| Databáze: | OpenAIRE |
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