Autoantibodies to GAD65 and IA-2 in canine diabetes mellitus
| Autor: | Brian Catchpole, M. E. Herrtage, Michael R. Christie, Sarah M. Weenink, L. J. Davison |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty endocrine system diseases medicine.medical_treatment Immunology Molecular Sequence Data Gene Expression GAD2 Dogs PTPRN Antigen Diabetes mellitus Internal medicine medicine Animals Receptor-Like Protein Tyrosine Phosphatases Class 8 Amino Acid Sequence Dog Diseases Cloning Molecular Insulinoma Autoantibodies General Veterinary biology business.industry Glutamate Decarboxylase Insulin Autoantibody medicine.disease Endocrinology Diabetes Mellitus Type 1 biology.protein Female Antibody business |
| Zdroj: | Veterinary immunology and immunopathology. 126(1-2) |
| ISSN: | 1873-2534 0165-2427 |
| Popis: | Diabetes mellitus in dogs shares many characteristics with the human type 1 disease and virtually all diabetic dogs require insulin therapy to control hyperglycaemia. Insulin deficiency is suspected to result from immune-mediated destruction of pancreatic beta cells in some cases. Human patients suffering from Type 1A (immune-mediated) diabetes or latent autoimmune diabetes of the adult (LADA) demonstrate circulating autoantibodies against the 65kDa isoform of glutamic acid decarboxylase (GAD65) and/or insulinoma antigen-2 (IA-2). The aims of the current study were to develop radio-immunoassays to detect serum antibodies against recombinant canine GAD65 and IA-2 and to identify diabetic dogs showing serological evidence of autoreactivity to these pancreatic beta cell antigens. Canine GAD65 and the 3' end of IA-2 (coding for amino acids 771-979 of the intracellular domain) were amplified by PCR from cDNA prepared from canine insulinoma tissue and cloned into the pCRII vector. The canine sequences were later confirmed by identifying GAD2 and PTPRN genes from the dog genome assembly. Recombinant (35)S-methionine-radiolabelled canine GAD65 and IA-2 (771-979) proteins were used in radio-immunoprecipitation assays to screen sera from 30 newly diagnosed diabetic dogs and 30 control dogs. Four of 30 canine diabetic patients had significant GAD65 autoreactivity (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect