hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
| Autor: | Anna Schueth, Alard Roebroeck, Sven Hildebrand, Klaus von Wangenheim, Francesco Pampaloni, Ralf A. W. Galuske, Christian Mattheyer, Hansjürgen Bratzke |
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| Přispěvatelé: | RS: FPN CN 11, Multiscale Imaging of Brain Connectivity |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Tissue Fixation
Swine lcsh:Medicine Neural circuits Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Optical clearing ddc:570 Formaldehyde Clearing medicine Animals Humans ddc:610 lcsh:Science Paraformaldehyde 030304 developmental biology Fixation (histology) Fluorescent Dyes 0303 health sciences Multidisciplinary Chemistry Lysine lcsh:R Periodic Acid Brain MICROSCOPY Affinity Labels Human brain Carbocyanines Lipids medicine.anatomical_structure lcsh:Q 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports, 10(1):9950. Nature Publishing Group Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance. |
| Databáze: | OpenAIRE |
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