Infrared imaging of high density protein arrays
Autor: | Erik Goormaghtigh, André Matagne, Julie Vandenameele, Joëlle De Meutter |
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Rok vydání: | 2016 |
Předmět: |
Infrared
Analytical chemistry Protein Array Analysis Infrared spectroscopy 02 engineering and technology 01 natural sciences Biochemistry Noise (electronics) Protein Structure Secondary Analytical Chemistry Optics Spectroscopy Fourier Transform Infrared Electrochemistry Image noise Image Processing Computer-Assisted Environmental Chemistry Protein secondary structure Spectroscopy Pixel business.industry Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Protein microarray 0210 nano-technology business |
Zdroj: | The Analyst. 142(8) |
ISSN: | 1364-5528 |
Popis: | We propose in this paper that protein microarrays could be analysed by infrared imaging in place of enzymatic or fluorescence labelling. This label-free method reports simultaneously a large series of data on the spotted sample (protein secondary structure, phosphorylation, glycosylation, presence of impurities, etc.). In the present work, 100 μm protein spots each containing about 100 pg protein were deposited to form high density regular arrays. Using arrays of infrared detectors, high resolution images could be obtained where each pixel of the image is in fact a full infrared spectrum. With microarrays, hundreds of experimental conditions can be tested easily and quickly, with no further labelling or chemistry of any kind. We describe how the noise present in the infrared spectra can be split into image noise and detector noise. We also detail how both types of noise can be most conveniently dealt with to generate very high quality spectra of less than 100 pg protein. Finally, the results suggest that the protein secondary structure is preserved during microarray building. |
Databáze: | OpenAIRE |
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