Effect of hyaluronidase on brain extracellular matrix in vivo and optic nerve regeneration
| Autor: | A. Tona, Amico Bignami |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Wallerian degeneration Cerebellum genetic structures Nerve Crush Central nervous system Receptors Lymphocyte Homing Hyaluronoglucosaminidase Receptors Cell Surface Rats Sprague-Dawley Cellular and Molecular Neuroscience Neurofilament Proteins Hyaluronidase Glial Fibrillary Acidic Protein medicine Animals Hyaluronic Acid Brain Chemistry Retina Glial fibrillary acidic protein biology Brain Optic Nerve Anatomy medicine.disease Axons eye diseases Extracellular Matrix Nerve Regeneration Rats Hyaluronan Receptors medicine.anatomical_structure biology.protein Optic nerve Female sense organs Carrier Proteins Astrocyte medicine.drug |
| Zdroj: | Journal of Neuroscience Research. 36:191-199 |
| ISSN: | 1097-4547 0360-4012 |
| DOI: | 10.1002/jnr.490360209 |
| Popis: | In the rat, intracerebral injection of bacterial hyaluronidase resulted in the almost complete disappearance of hyaluronic acid (HA) and glial hyaluronate-binding protein (GHAP) from cerebral hemispheres, brain stem, and cerebellum (but not from optic nerves and chiasm) starting 2–3 hr after the injection. HA and GHAP reappeared throughout the brain in characteristic patches 2–3 days after the injection. The patches gradually became confluent and after 12 days the brain appeared virtually normal. In normal rat optic nerve, staining for HA and GHAP ceased abruptly in the region of the lamina cribrosa. The retina was completely negative. HA and GHAP disappeared from hyaluronidase-injected optic nerve, chiasm, and contralateral optic nerve. In hyaluronidase-injected crushed optic nerves, regenerated axons were able to grow for short distances (about 500μm) into the distal stump undergoing Wallerian degeneration. No such growth was observed in saline-injected controls. © 1993 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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