Deep phenotyping and biomarkers of various dairy fat intakes in an 8-week randomized clinical trial and 2-year swine study

Autor: Jean-Charles Martin, Claire Bal-dit-Sollier, Jean-Marie Bard, Denis Lairon, Michel Bonneau, Chantal Kang, Murielle Cazaubiel, Corinne Marmonier, Pascale Leruyet, Constance Boyer, Hassan Nazih, Catherine Tardivel, Catherine Defoort, Marion Pradeau, Imene Bousahba, Habib Hammou, Ljubica Svilar, Ludovic Drouet
Přispěvatelé: Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de Cancérologie de l'Ouest [Angers/Nantes] (UNICANCER/ICO), UNICANCER, Sciences de l'Animal et de l'Aliment de Jouy (SAAJ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biofortis, Centre National Interprofessionnel de l'Economie Laitière [Paris] (CNIEL), LACTALIS RECHERCHE ET DEVELOPPEMENT (LACTALIS R&D), Groupe Lactalis, Université d'Oran 1 Ahmed Ben Bella [Oran]
Rok vydání: 2022
Předmět:
Zdroj: Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry, 2023, 113, pp.109239. ⟨10.1016/j.jnutbio.2022.109239⟩
ISSN: 1873-4847
0955-2863
DOI: 10.1016/j.jnutbio.2022.109239⟩
Popis: International audience; Health effects of dairy fats (DF) are difficult to evaluate, as DF intakes are hard to assess epidemiologically and DF have heterogeneous compositions that influence biological responses. We set out to find biomarkers of DF intake and assess biological response to a summer DF diet (R2), a winter DF diet (R3), and a R3 supplemented with calcium (R4) compared to a plant-fat-based diet (R1) in a randomized clinical trial (n=173) and a 2-year study in mildly metabolically disturbed downsized pigs (n=32). Conventional clinical measures were completed by LC/MS plasma metabolomics/lipidomics. The measured effects were modeled as biological functions to facilitate interpretation.DF intakes in pigs specifically induced a U-shaped metabolic trajectory, reprogramming metabolism to close to its initial status after a one-year turnaround. Twelve lipid species repeatably predicted DF intakes in both pigs and humans (6.6% errors). More broadly, in pigs, quality of DF modulated the time-related biological response (R2: 30 regulated functions, primarily at 6 months; R3: 26 regulated functions, mostly at 6–12 months; R4: 43 regulated functions, mostly at 18 months). Despite this heterogeneity, 9 functions overlapped under all 3 DF diets in both studies, related to a restricted area of amino acids metabolism, cofactors, nucleotides and xenobiotic pathways and the microbiota. In conclusion, over the long-term, DF reprograms metabolism to close to its initial biological status in metabolically-disrupted pigs. Quality of the DF modulates its metabolic influence, although some effects were common to all DF. A resilient signature of DF consumption found in pigs was validated in humans.
Databáze: OpenAIRE
Externí odkaz: