Analysis of the antigenic relationship of various derivatives of n-acetyl-muramyl-l-ala-d-isoglutamine (MDP), using anti-MDP antibodies
| Autor: | Claude Carelli, F. Z. Modabber, Louis Chedid, Francoise Audibert, George M. Bahr |
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| Rok vydání: | 1982 |
| Předmět: |
Male
Isoglutamine Antigenicity Immunology Enzyme-Linked Immunosorbent Assay Conjugated system Binding Competitive chemistry.chemical_compound Antigen Antibody Specificity parasitic diseases Animals Antigens Molecular Biology biology Chemistry Glycopeptides Biological activity Glycopeptide body regions Biochemistry biology.protein Rabbits Antibody Hapten Acetylmuramyl-Alanyl-Isoglutamine Haptens |
| Zdroj: | Molecular immunology. 19(5) |
| ISSN: | 0161-5890 |
| Popis: | Antibodies to N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP) were produced in rabbits by injection of MDP conjugated to various carriers [bovine gamma globulin (BGG), methylated BSA or sheep erythrocytes]. The anti-MDP was assayed by a direct enzyme-linked immunosorbent assay (ELISA) using horse radish peroxidase linked to MDP-Lys. Various derivatives of MDP were employed in an inhibition of ELISA for analysis of specificity of antibodies and study of the relationship of configuration to biological activity. The results confirmed previous findings that MDP alone is not immunogenic but can act as a hapten when conjugated to carriers. The antibodies were shown to be primarily directed against the muramyl residue. Modifications of this region of MDP yielded derivatives with weak reactivity against anti-MDP, while some changes of other regions had no effect on its antigenicity. Optical isomers of MDP had reduced activity as compared to MDP and polymeric MDP was a strong inhibitor. The structure and function relationship is discussed for some derivatives. |
| Databáze: | OpenAIRE |
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