Differential induction of enzymes involved in anaerobic metabolism of aromatic compounds in the denitrifying bacterium Thauera aromatica
| Autor: | Magdy El-Said Mohamed, Matthias Boll, Johann Heider, Georg Fuchs, Birgitta Leuthner, Nasser Gad'on, S. Breinig, Klaus Breese, U. Feil, G. Burchhardt, Diana Laempe, Christa Ebenau-Jehle, Sabine Schneider |
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| Rok vydání: | 1998 |
| Předmět: |
Oxidoreductases Acting on CH-CH Group Donors
Thauera aromatica Phenylalanine Reductase Benzoates Hydrocarbons Aromatic Biochemistry Microbiology Bacterial Proteins Phenols Coenzyme A Ligases Genetics Coenzyme A Electrophoresis Gel Two-Dimensional Anaerobiosis Molecular Biology Phenylacetates chemistry.chemical_classification DNA ligase Bacteria biology General Medicine Metabolism biology.organism_classification Enzyme assay Metabolic pathway Enzyme chemistry Benzoyl-CoA reductase Enzyme Induction biology.protein Acyl Coenzyme A Oxidoreductases Toluene |
| Zdroj: | Archives of Microbiology. 170:120-131 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s002030050623 |
| Popis: | Differential induction of enzymes involved in anaerobic metabolism of aromatic substrates was studied in the denitrifying bacterium Thauera aromatica. This metabolism is divided into (1) peripheral reactions transforming the aromatic growth substrates to the common intermediate benzoyl-CoA, (2) the central benzoyl-CoA pathway comprising ring-reduction of benzoyl-CoA and subsequent beta-oxidation to 3-hydroxypimelyl-CoA, and (3) the pathway of beta-oxidation of 3-hydroxypimelyl-CoA to three acetyl-CoA and CO2. Regulation was studied by three methods. 1. Determination of protein patterns of cells grown on different substrates. This revealed several strongly substrate-induced polypeptides that were missing in cells grown on benzoate or other intermediates of the respective metabolic pathways. 2. Measurement of activities of known enzymes involved in this metabolism in cells grown on different substrates. The enzyme pattern found is consistent with the regulatory pattern deduced from simultaneous adaptation of cells to utilisation of other aromatic substrates. 3. Immunological detection of catabolic enzymes in cells grown on different substrates. Benzoate-CoA ligase and 4-hydroxybenzoate-CoA ligase were detected only in cells yielding the respective enzyme activity. However, presence of the subunits of benzoyl-CoA reductase and 4-hydroxybenzoyl-CoA reductase was also recorded in some cell batches lacking enzyme activity. This possibly indicates an additional level of regulation on protein level for these two reductases. |
| Databáze: | OpenAIRE |
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