Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK
| Autor: | Li-Oon Chuah, Kien-Pong Yap, Min-Tze Liong, Sy Bing Choi, Lee-Ching Lew, Boon-Kiat Lee, Azali Azlan, Ghows Azzam, Yan-Yan Hor, Amy-Sie-Yik Lau, M.H. Jaafar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
AMPK
medicine.medical_specialty Statin Normal diet medicine.drug_class ABCG8 Article Catalysis lcsh:Chemistry Inorganic Chemistry chemistry.chemical_compound NAFLD Internal medicine Hyperlipidemia medicine triglyceride Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Triglyceride biology fungi Organic Chemistry nutritional and metabolic diseases food and beverages General Medicine medicine.disease Computer Science Applications SCD1 Lactobacillus Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry ABCG5 biology.protein lipids (amino acids peptides and proteins) Lovastatin immune medicine.drug |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 5872, p 5872 (2020) International Journal of Molecular Sciences Volume 21 Issue 16 |
| ISSN: | 1422-0067 |
| Popis: | In this study, we hypothesized that different strains of Lactobacillus can alleviate hyperlipidemia and liver steatosis via activation of 5&prime adenosine monophosphate-activated protein kinase (AMPK), an enzyme that is involved in cellular energy homeostasis, in aged rats. Male rats were fed with a high-fat diet (HFD) and injected with D-galactose daily over 12 weeks to induce aging. Treatments included (n = 6) (i) normal diet (ND), (ii) HFD, (iii) HFD-statin (lovastatin 2 mg/kg/day), (iv) HFD-Lactobacillus fermentum DR9 (10 log CFU/day), (v) HFD-Lactobacillus plantarum DR7 (10 log CFU/day), and (vi) HFD-Lactobacillus reuteri 8513d (10 log CFU/day). Rats administered with statin, DR9, and 8513d reduced serum total cholesterol levels after eight weeks (p < 0.05), while the administration of DR7 reduced serum triglycerides level after 12 weeks (p < 0.05) as compared to the HFD control. A more prominent effect was observed from the administration of DR7, where positive effects were observed, ranging from hepatic gene expressions to liver histology as compared to the control (p < 0.05) downregulation of hepatic lipid synthesis and &beta oxidation gene stearoyl-CoA desaturase 1 (SCD1), upregulation of hepatic sterol excretion genes of ATP-binding cassette subfamily G member 5 and 8 (ABCG5 and ABCG8), lesser degree of liver steatosis, and upregulation of hepatic energy metabolisms genes AMPK&alpha 1 and AMPK&alpha 2. Taken altogether, this study illustrated that the administration of selected Lactobacillus strains led to improved lipid profiles via activation of energy and lipid metabolisms, suggesting the potentials of Lactobacillus as a promising natural intervention for alleviation of cardiovascular and liver diseases. |
| Databáze: | OpenAIRE |
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