Biomechanical properties of veins cultured in vitro under elevated internal pressure
| Autor: | Masuya Kurimoto, Kozaburo Hayashi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pathology
medicine.medical_specialty Vascular smooth muscle Physiology 0206 medical engineering Femoral vein Blood Pressure 02 engineering and technology Vascular Remodeling 030204 cardiovascular system & hematology Tissue Culture Techniques Contractility 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine In vivo Physiology (medical) medicine Animals Vein Mechanical Phenomena business.industry Histology Femoral Vein 020601 biomedical engineering In vitro Biomechanical Phenomena medicine.anatomical_structure Rabbits business |
| Zdroj: | Biorheology. 55:25-40 |
| ISSN: | 1878-5034 0006-355X |
| Popis: | BACKGROUND The venous response to elevated blood pressure (BP) is of major importance because it is closely related to the etiology of venous diseases and the competency of vein grafts. In vitro culture experiments may provide useful information on the function of vein grafts because it is easier to separate mechanical and hemodynamic effects from other systemic influences compared to in vivo experiments. OBJECTIVE To study the effects of BP elevation on wall dimensions and mechanical properties of in vitro cultured veins. METHODS Rabbit femoral veins were cultured in vitro under internal pressures of 1 to 50 mmHg for 1 week, and their wall dimensions, biomechanical properties, and histology were determined. RESULTS No significant differences were observed in internal vein diameter and wall thickness among vessels cultured at 10-50 mmHg compared to non-cultured control vessels. For an internal pressure of 10 mmHg applied to vessels during culture (equivalent to in vivo working BP), wall circumferential stress was maintained within control levels. There were no significant effects of pressure on basal tone and contractility of vascular smooth muscle and vascular compliance. CONCLUSIONS The in vitro results were essentially similar to those obtained from previous in vivo animal experiments, indicating that in vitro tissue culture techniques are applicable to studies of venous remodeling. |
| Databáze: | OpenAIRE |
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