The photoaffinity probe 8-N3 [α-32 P]ATP labels the ATP-binding sites of rabbit neutrophil and skeletal muscle actin
| Autor: | Chi-Kuang Huang, Elmer L. Becker, Barbara-Jean Bormann, William M. Mackin, John M. Hill |
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| Rok vydání: | 1983 |
| Předmět: |
Azides
Neutrophils Photochemistry Biophysics Biochemistry Adenosine Triphosphate Structural Biology Photoaffinity labeling Genetics medicine Animals Isoelectric Point Binding site Molecular Biology Actin Binding Sites Molecular mass Chemistry Skeletal muscle Rabbit (nuclear engineering) Cell Biology Actins Subcellular distribution Isoelectric point medicine.anatomical_structure Rabbits 8-N3-ATP Subcellular Fractions |
| Zdroj: | FEBS Letters. 159:145-149 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(83)80434-7 |
| Popis: | 8-Azido-[α- 32 P]ATP (8-N 3 -ATP) was used as a photoaffinity label for ATP binding sites in the subcellular fractions of rabbit peritoneal neutrophils. The radioactive 8-N 3 -ATP was specifically incorporated into one major protein of 43 kDa. The isoelectric point, molecular mass and subcellular distribution of this labeled protein closely resemble those of the actin. 8-N 3 -[α- 32 P]ATP was further tested as a photoaffinity label for the ATP binding site in the purified rabbit skeletal muscle G-actin. The radioactive 8-N 3 -ATP was specifically incorporated into the actin band in SDS—polyacrylamide gel. The results indicate that 8-N 3 -ATP can be used as a photoaffinity label for actin. |
| Databáze: | OpenAIRE |
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