Integrative vectors for gene deletion and replacement
| Autor: | Magdalena Kucharska, Ewa Jodelko, Marian Sektas, Magdalena Gregorowicz |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Genetics Cloning DNA Bacterial FLP-FRT recombination Gene targeting Chromosome General Medicine Gene Expression Regulation Bacterial Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Plasmid chemistry Mutagenesis medicine Homologous chromosome Escherichia coli DNA Gene Deletion |
| Zdroj: | Polish journal of microbiology. 62(1) |
| ISSN: | 1733-1331 |
| Popis: | A b s t r a c t An improved method for gene deletion or replacement in Escherichia coli was developed. It employs a set of integrative vectors and two helper plasmids, as a temporary source of RecA and Flp activities. #e integrative vectors combine several useful features including three di%erent selection markers placed between two parallel oriented Flp recombinase target ( FRT ) sites. Each marker is $anked by two MCSs, for cloning the chosen homologous fragments of DNA to gene targeting. #e vectors contain two properly oriented E. coli Chi sites for recombination enhancement. When required, selection markers can be excised from the chromosome resulting in unmarked strains. K e y w o r d s: gene deletion; gene replacement, integrative rectors |
| Databáze: | OpenAIRE |
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