Murine Full-thickness Skin Transplantation
| Autor: | Jonathan D. Powell, Chih Hsien Cheng, Madeline Fryer, Georg J. Furtmüller, Byoung Chol Oh, Chen Fang Lee, Gerald Brandacher |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Graft Rejection medicine.medical_specialty Graft failure General Chemical Engineering Immunology Full thickness skin transplantation Palpation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Medicine Animals Transplantation Homologous Sirolimus Mice Inbred BALB C Respiratory distress medicine.diagnostic_test Graft rejection General Immunology and Microbiology business.industry General Neuroscience Skin Transplantation Skin transplantation Surgery Transplantation Mice Inbred C57BL 030104 developmental biology surgical procedures operative Cyclosporine business Bandage Immunosuppressive Agents |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/55105-v |
| Popis: | Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited application to humans, skin transplantation in mice has been widely employed for transplantation research. The procedure is easy to learn and perform, and it does not require delicate microsurgical techniques nor extensive training. Moreover, graft rejection in this model occurs in a very reproducible immunological reaction and is easily monitored by direct inspection and palpation. In addition, secondary skin transplantation with donor-matched or third-party skin grafts can be performed on more complex transplant models as an alternative and uncomplicated method to assess donor-specific tolerance. The complications are low and are in general limited to anesthesia overdose or respiratory distress after the procedure. Graft failure, on the other hand, occurs commonly as a result of poor preparation of the graft, incorrect positioning in the graft bed, or inappropriate placement of the bandage. In this article, we present a protocol for full-thickness skin transplantation in mice and describe the important steps necessary for a successful procedure. |
| Databáze: | OpenAIRE |
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