Altered TH1, TH2, TH17 balance in assisted reproductive technology conceived mice
| Autor: | Fardin Amidi, Aligholi Sobhani, Ashraf Moeini, Shayesteh Mehdinejadiani, Shirzad Hoseini, Mojdeh bahram rezai, Fardin Fathi, Hamid Ahmadi, Hiwa Karimi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Reproductive Techniques Assisted Offspring medicine.medical_treatment Immunology Mice Inbred Strains Reproductive technology Fertilization in Vitro Intracytoplasmic sperm injection Andrology 03 medical and health sciences Interferon-gamma Mice 0302 clinical medicine Immune system Th2 Cells medicine Immunology and Allergy Animals Humans Sperm Injections Intracytoplasmic Th1-Th2 Balance Cells Cultured Mycobacterium bovis Immunity Cellular 030219 obstetrics & reproductive medicine In vitro fertilisation Assisted reproductive technology biology business.industry Obstetrics and Gynecology Th1 Cells biology.organism_classification 030104 developmental biology Reproductive Medicine Immunization Th17 Cells Female business T-Box Domain Proteins |
| Zdroj: | Journal of reproductive immunology. 139 |
| ISSN: | 1872-7603 |
| Popis: | Aim and purpose The higher prevalence rate of different diseases may accentuate the possible alteration of the immune system in individuals conceived through the assisted reproductive technologies (ART). The present study was conducted to evaluate the TH1, TH2, TH17 balance in intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) - conceived mice in comparison to naturally conceived offspring. Methods Mice (6-8 weeks) were divided into three groups (IVF- conceived, ICSI- conceived and naturally conceived). They were subjected to subcutaneous immunization with Mycobacterium bovis Bacille Calmette-Guerin (BCG). The blood samples were taken and the sera were separated. Then the spleens were surgically removed at the time the mice were sacrificed. Serum levels of IFN-γ, IL-17A and IL-4 were detected by ELISA. Then the proportion of TCD4 cells possessing the T-bet+, GATA3+, and ROR-γt + were measured using FACS caliber flow cytometer. Results In comparison with naturally conceived mice, intracellular expression of T-bet and serum levels of IFN-γ were significantly decreased in ART- conceived mice. Moreover, ART- conceived offspring showed marked increase in IL-4 and IL-17A. Conclusion It is concluded that compared to naturally conceived male mice, ART- conceived counterparts exhibit less efficient immune responses against BCG through further promotion of humoral and inflammatory related immune response characteristics. |
| Databáze: | OpenAIRE |
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